Sampling and Selective Detection of Peptides and Proteins Emphazing Microdialysis,Bio-recognition Assays and MALDI-TOF MS

Detta är en avhandling från Analytical Chemistry (S/LTH)

Författare: Sven Kjellström; Lunds Universitet.; Lund University.; [2000]

Nyckelord: NATURVETENSKAP; NATURAL SCIENCES; protein identification cyclodextrines m-dispenser push-pull microdialysis microdialysis sampling MALDI-TOF MS Interleukin-8 Flow immuno assays Interleukin-10 Analytical chemistry Analytisk kemi;

Sammanfattning: The use of microdialysis sampling and flow immunochemical detection as well as MALDI-TOF MS for peptide and protein analysis is described. Sampling of peptides and smaller proteins was investigated with different membranes. Cyclodextrines additives and an on-line push-pull microdialysis sampling method was used with the result of a higher sampling efficiency compared to conventional microdialysis sampling. Real-time flow immuno assays systems were developed for the quantification of Interleukin-8 and Interleukin10 in cell samples. Optimisation with regards to affinity support selection as well as carrier buffer composition were performed. Connection of capillary LC with MALDI-TOF MS was made utilising an m-dispenser technique enabling sample handling of picoliter volumes. A novel sample preparation technique for the uniform coating of MALDI targets was developed.

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