Sökning: "Jose-Luis Millan"

Hittade 1 avhandling innehållade orden Jose-Luis Millan.

1. 1. Structural / functional studies on human alkaline phosphatases

   Författare :Alexey Kozlenkov; Jose-Luis Millan; Hans Jörnvall; Umeå universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biomedicine; mutagenesis; enzyme kinetics; computer modeling; crystallography; natural substrates; uncompetitive inhibition; isozymes; gene families; Biomedicin; Microbiology; Mikrobiologi; medicinsk genetik; Medical Genetics;

   Sammanfattning : The recent elucidation of the three-dimensional structure of human placental alkaline phosphatase (PLAP) has enabled me to perform structural studies aimed at characterizing the properties of human PLAP and tissue-nonspecific AP (TNAP) as paradigms for mammalian APs in general, using site-directed mutagenesis, protein expression, kinetic analysis and computer modeling. In Paper I, we found that a single critical E429G substitution explains the difference in stability and kinetics between the common allelic variants of PLAP and the D allozyme. LÄS MER