Sökning: "size-exclusion chromatography"

Visar resultat 1 - 5 av 61 avhandlingar innehållade orden size-exclusion chromatography.

  1. 1. Characterizing Chromatography Media : NMR-based Approaches

    Författare :Fredrik Elwinger; István Furó; Stéphane Viel; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; chromatography; nuclear magnetic resonance; NMR; self-diffusion; magic-angle spinning; quantitative; distribution coefficient; pore size; Kemi; Chemistry;

    Sammanfattning : Liquid chromatography is an essential technique in manufacturing biopharmaceuticals where it is used on all scales from analytical applications in R&D to full-scale production. In chromatography the target molecule, typically a protein, is separated and purified from other components and contaminants. LÄS MER

  3. 2. Modeling Antibody Aggregation in Downstream Processing

    Författare :Frida Ojala; Avdelningen för kemiteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Antibody; Aggregation; Downstream processing; Size exclusion chromatography; Reversible reaction model; General rate model;

    Sammanfattning : Antibodies are part of the immune system where their purpose is to recognize and neutralize foreign species such as viruses and bacteria, thus preventing and curing infections. The trend of using antibodies in the treatment of, for example, cancer is increasing. LÄS MER

  4. 3. Isolation, characterization and enzymatic hydrolysis of water-soluble wood polysaccharides

    Författare :Jon Lundqvist; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; size exclusion chromatography; beta-mannosidase; beta-mannanase; endoglucanase; ethyl hydoxyethyl cellulose; metabolism; Biokemi; Biochemistry; Metabolism; microwave irradiation; O-acetyl-galactoglucomannan; isolation;

    Sammanfattning : The need for new renewable products has enhanced the interest in cellulose and hemicellulose as raw material. The wood plant cell wall is a complex mixture of cellulose, hemicellulose, lignin and minor amounts of pectin, proteins and extractives. Several interactions between the compounds make them difficult to separate without modification. LÄS MER

  5. 4. Cellulose degradation in pulp fibers studied as changes in molar mass distributions

    Författare :Rickard Berggren; KTH; []
    Nyckelord :cellulose; kraft pulp; birch; spruce; ozonation; acid hydrolysis; degradation; MMD; size-exclusion chromatography; light scattering; molar mass; chain scission;

    Sammanfattning : In this thesis, size-exclusion chromatography (SEC) of woodpolymers dissolved in lithium chloride/N,N-dimethylacetamide(LiCl/DMAc) has been used to characterize the molar massdistributions (MMD) of wood polymers in pulp fibers afterchemical degradation.Characterization of birch kraft pulps subjected to ozonedegradation and acid hydrolysis, respectively, rendereddifferent changes in the MMD. LÄS MER

  7. 5. Hemicelluloses from Agricultural and Forestry Crops Isolation, Characterisation, and Enzymatic Hydrolysis

    Författare :Alexandra Andersson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; Physical chemistry; Fysikalisk kemi; enzymology; hydrogels; Plant biochemistry; Växtbiokemi; Proteins; a-galactosidase; b-mannanase; hemicellulase; corn fibre; barley husks; spruce; size-exclusion chromatography; arabinoxylan; microwave irradiation; steam pretreatment; membrane filtration; hemicellulose; galactoglucomannan;

    Sammanfattning : Hemicelluloses are tremendous sources of renewable raw materials, which utilisation have the potential to be highly increased in the future. Hemicelluloses are among the major groups of compounds in plant cell walls, together with cellulose and lignin. These three components are closely associated, making them difficult to separate from each other. LÄS MER