Sökning: "total internal reflection fluorescence microscopy"

Visar resultat 6 - 10 av 20 avhandlingar innehållade orden total internal reflection fluorescence microscopy.

  1. 6. Waveguide Evanescent-Field Microscopy for Label-Free Monitoring of Biological Nanoparticles: Fabrication, Characterization and Application

    Författare :Mokhtar Mapar; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Biosensor; Protein interaction; Waveguide scattering microscopy; Waveguide fluorescence microscopy; Supported lipid bilayer; Image processing; Lipid vesicle; Nanofabrication; Exosome;

    Sammanfattning : The recent development of microscopy methods, biological assays and bioanalytical sensors has significantly advanced the understanding of biological systems. Surface-based bioanalytical sensors have in recent years gained increased interest thanks to improvements in sensitivity and simplicity to use. LÄS MER

  3. 7. Unraveling the molecular mechanisms of herpes simplex virus attachment and release using cell membrane mimics

    Författare :Nadia Peerboom; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; total internal reflection fluorescence microscopy; glycosaminoglycans; atomic force microscopy; binding kinetics; single particle tracking; herpes simplex virus;

    Sammanfattning : The herpes simplex virus is a widespread human pathogen, most commonly known for causing cold sores. Its infection cycle is initiated with the formation of multiple bonds between viral glycoproteins and cellular glycosaminoglycans, which are long polysaccharide chains found close to the cell surface. LÄS MER

  4. 8. Caveolae associated proteins and how they effect caveolae dynamics

    Författare :Björn Morén; Richard Lundmark; Volker Haucke; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Caveolae; caveolin; EHD2; cavin; microdomain; microscopy; TIRF; AFM; medicinsk biokemi; Medical Biochemistry;

    Sammanfattning : Caveolae are a type of invaginated membrane domain that has been shown to be involved in several disease states, including lipodystrophy, muscular dystrophies and cancer. Several of these diseases are caused by the lack of caveolae or caveolae-related signaling deficiencies in the tissues in which the caveolar domain are abundant such as lung, adipose, muscle and their related endothelial cells. LÄS MER

  5. 9. Supported Lipid Membranes and Their Use for the Characterization of Biological Nanoparticles

    Författare :Silver Jõemetsa; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; fluorescence microscopy; supported lipid bilayer; lipophilic dyes; size determination; diffusion; microfluidics;

    Sammanfattning : Biological nanoparticles (BNPs) are nano-sized lipid vesicles of biological origin, which are involved in multiple biological processes. BNP characterization techniques are critical for improving the understanding of how these particles contribute to cellular communication, viral infections and drug-delivery applications. LÄS MER

  7. 10. Molecular mechanisms of biphasic insulin secretion

    Författare :Nikhil R. Gandasi; Sebastian Barg; Christien Merrifield; Uppsala universitet; []
    Nyckelord :Exocytosis; Insulin secretion; Membrane trafficking; Microscopy; Diabetes; Medical Cell Biology; Medicinsk cellbiologi;

    Sammanfattning : Pancreatic beta-cells secrete insulin in response to increase in blood glucose concentration with a rapid first phase and slower, sustained second phase. This secretion pattern is similar in entire pancreas, isolated islets of Langerhans and single beta-cells and it is disrupted in type 2-diabetes. LÄS MER