Avancerad sökning

Hittade 5 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Evolution of streamlined genomes in ultra-small aquatic bacteria

   Författare :Weizhou Zhao; Siv Andersson; Sandra L. Baldauf; Sarahi L Garcia; Karen Lloyd; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; streamlined genome; aquatic bacteria; single cell genomics; metagenomics; evolutionary genomics; salinity; Baltic Sea; Biologi med inriktning mot molekylär evolution; Biology with specialization in Molecular Evolution;

   Sammanfattning : This thesis investigates the evolutionary processes of streamlined genomes from aquatic bacteria adapting to different salinities, using two groups of ultra-small aquatic bacteria (LD12 Alphaproteobacteria and acI Actinobacteria). Due to difficulties in obtaining pure cultures of these bacteria, culture-free approaches (single-cell genomics and metagenomics) were used to construct and compare genomes, and to study the mechanisms and selective forces of adaptation to freshwater, brackish, and marine ecosystems. LÄS MER

3. 2. Exposing the Dark Microbial Biosphere

   Författare :Valerie Hubalek; Stefan Bertilsson; Alexander Eiler; Tim Urich; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : Dark biosphere research has been widely neglected, although by volume this biome comprises the lion’s share of habitats on our planet. In these systems the main metabolic strategies are of chemotrophic nature, leading to gradual depletion of redox gradients essential for sustaining life. LÄS MER

4. 3. From Tissue to Mutations : Genetic Profiling of Colorectal Cancer

   Författare :Lucy Mathot; Tobias Sjöblom; Mats Nilsson; Alberto Bardelli; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Nucleic acid extraction; genotyping; targeted sequencing; mutation analysis; colorectal cancer; metastatic disease; Medical Science; Medicinsk vetenskap;

   Sammanfattning : Comprehensive characterisation of the mutational landscapes of solid tumours is a multistep process involving the collection of suitable samples, the extraction of nucleic acids and the preparation of these materials for mutational analyses. In this thesis, I aimed to develop a streamlined process for the analysis of colorectal cancer (CRC) patient samples in order to identify novel mutations that hallmark the development of advanced disease. LÄS MER

5. 4. Systems enabling antibody-mediated proteomics research

   Författare :Ronny Falk; Sophia Hober; Christer Wingren; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Antibody generation; dual expression; affinity purification; E. coli expression; Affibody molecules; polyclonal antibody; monospecific antibody; Immunobiology; Immunbiologi;

   Sammanfattning : As many genome sequencing efforts today are completed, we are now provided with the genetic maps for several organisms, including man. With these maps at hand, the scientific focus is now shifting towards investigations of the functionality of proteins. LÄS MER

7. 5. Carbon metabolism in non-conventional yeasts: biodiversity, origins of aerobic fermentation and industrial applications

   Författare :Nerve Zhou; Molekylär cellbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aerobic fermentation; carbon metabolism; non-conventional yeasts; strain development;

   Sammanfattning : Abstract: For millennia, the “yeast” Saccharomyces cerevisiae remains by far the most extensively studied and exploited yeast in food and industrial applications. A number of researches and developments have been done since the establishment of the biochemical function of yeast by Louis Pasteur in 1860, however modern lifestyles often connected to food related health trends demand new and innovative food products. LÄS MER