Sökning: "Antibody generation"

Visar resultat 1 - 5 av 117 avhandlingar innehållade orden Antibody generation.

  1. 1. Generation and evolution of human antibody repertoires

    Författare :Helena Persson; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; specificity; Immunology; serology; transplantation; Immunologi; serologi; Biotechnology; Bioteknik; phage display; IgE; hapten; grass pollen allergen; diversity; antibody repertoire; antibody library; antibody evolution; affinity maturation; allergy;

    Sammanfattning : This thesis, which is based on five original papers, covers different aspects of antibody specificity and diversity, with the overall goal to understand the makeup of antibody repertoires and their functionality as they occur in vivo or as they are developed in the laboratory. Besides their importance in the adaptive immune response, antibodies have emerged as an invaluable tool in applications ranging from basic research to disease diagnostics and therapy. LÄS MER

  3. 2. Systems enabling antibody-mediated proteomics research

    Författare :Ronny Falk; Sophia Hober; Christer Wingren; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Antibody generation; dual expression; affinity purification; E. coli expression; Affibody molecules; polyclonal antibody; monospecific antibody; Immunobiology; Immunbiologi;

    Sammanfattning : As many genome sequencing efforts today are completed, we are now provided with the genetic maps for several organisms, including man. With these maps at hand, the scientific focus is now shifting towards investigations of the functionality of proteins. LÄS MER

  4. 3. Generation and characterization of antibodies for proteomics research

    Författare :Karin Larsson; Sophia Hober; John McCafferty; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody; antibody validation; immunohistochemistry; immunofluorescence; tissue microarray; Western blot; epitope mapping; antigen design; Bioengineering; Bioteknik;

    Sammanfattning : Specific antibodies are invaluable tools for proteomics research. The availability of thoroughly validated antibodies will help to improve our understanding of protein expression, localization and function; fundamental processes and features of all living organisms. LÄS MER

  5. 4. Selection of antigens for antibody-based proteomics

    Författare :Lisa Berglund; Mathias Uhlén; Sören Brunak; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; epitope; antigen; antibody; affinity; protein; proteome; proteomics; bioinformatics; prediction; primer design; sequence similarity; Bioengineering; Bioteknik;

    Sammanfattning : The human genome is predicted to contain ~20,500 protein-coding genes. The encoded proteins are the key players in the body, but the functions and localizations of most proteins are still unknown. LÄS MER

  7. 5. Methods for Generation and Characterization of Monospecific Antibodies

    Författare :Johan Rockberg; Mathias Uhlén; John McCafferty; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody; epitope mapping; HER2; human protein atlas; immunogenicity; proteomics; Bioengineering; Bioteknik;

    Sammanfattning : Recent advances in biotechnology have generated possibilities to investigate and measure parts of life previously left for believers to explain. Utilizing the same book of recipes, the genome, our cells produce selections of proteins at a time and thereby niche into a multitude of specialized cell types, tissues and organs comprising our body. LÄS MER