Sökning: "slices"

Visar resultat 11 - 15 av 129 avhandlingar innehållade ordet slices.

1. 11. N-acetylaspartate in brain - studies on efflux and function

   Författare :Mattias Tranberg; Göteborgs universitet; []
   Nyckelord :N-acetylaspartate; Hippocampal slice cultures; HPLC; NMDA; Excitotoxicity; Volume regulation; Canavan disease;

   Sammanfattning : N-acetylaspartate (NAA) is an amino acid derivative present in high concentration in the brain. The function of NAA is still unsettled in spite of 50 years of research. The mainly neuronal synthesis and glial breakdown of NAA requires a well regulated neuronal efflux and glial uptake. LÄS MER

3. 12. Adrenal Bioactivation and Toxicity of 3-MeSO2-DDE, o,p´-DDD and DMBA Investigated in Tissue Slice Culture

   Författare :Örjan Lindhe; Jan Alexander; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Developmental biology; adrenal cortex; 3-MeSO2-DDE; o; p´-DDD; DMBA; bioactivation; binding; toxic; Utvecklingsbiologi; Developmental biology; Utvecklingsbiologi; Ecotoxicology; ekotoxikologi;

   Sammanfattning : I developed a precision-cut adrenal slice culture procedure to investigate cytochrome P450 (CYP) catalysed irreversible binding and adrenocorticolytic effects in human, rodent, and fish adrenal tissue, ex vivo. Autoradiography and radioluminography of exposed tissue slices showed that the potent adrenal toxicant 3-methylsulphonyl-2,2´-bis(4-chlorophenyl)-1,1´-dichloroethene (MeSO2-DDE) causes a selective metabolite binding in zona fasciculata (ZF), which is diminished by the CYP11B1 inhibitor metyrapone. LÄS MER

4. 13. High-Tc SQUIDs for magnetophysiology - development of a magnetometer system and measurements of evoked fields from hippocampal neurons in vitro

   Författare :Per Magnelind; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; neuromagnetism; brain slice; neurophysiology; electrophysiology; hippocampus; biomagnetism; SQUID;

   Sammanfattning : This thesis concerns development of a system based on a high-transition-temperature superconducting quantum interference device (high-Tc SQUID) magnetometer. The system has been designed and used for magnetic field recordings from brain slices, i.e. magnetophysiology. LÄS MER

5. 14. Microfluidics based techniques for electrophysiological studies of cells

   Författare :Aikeremu Ahemaiti; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : This thesis work investigates the application of microfluidics to perform electrophysiological studies on cells, including investigations of the effect of cholesterol on the dynamic ion permeability of TRPV1 ion channels, and the application of a microfluidic device, the multifunctional pipette, in electrophysiological studies on brain slices. In the first part of this thesis, Chinese hamster ovary (CHO) cells overexpressing the TRPV1 ion channel were used in a dynamic ion permeability study, where the activation properties of the TRPV1 ion channel were investigated using the patch clamp technique after depletion of membrane cholesterol. LÄS MER

7. 15. Pancreatic cancer : investigation of the prognostic significance of tumor immunophenotype and establishment of a novel ex-vivo tissue slice culture system for drug sensitivity testing

   Författare :Carlos Fernández Moro; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Pancreatic ductal adenocarcinoma (PDAC), commonly referred to as pancreatic cancer, is associated with a dismal 5-year overall survival of 9 to 6 %. A major reason for the high mortality is the pronounced resistance to treatment. LÄS MER