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1. 1. Poly(A)-Specific Ribonuclease (PARN)

   Författare :Yan-Guo Ren; Eric Westhof; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; Poly A -specific ribonuclease; PARN; deadenylation; processive; oligomeric; cap interacting; aminoglycoside; active site; FE2 -mediated cleavage; metal ion switch; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; Molekylär cellbiologi; Molecular Cellbiology;

   Sammanfattning : Degradation of the mRNA 3'-end located poly(A) tail is an important step for mRNA decay in mammalian cells. Thus, to understand mRNA decay in detail, it is important to identify the catalytic activities involved in degrading poly(A). LÄS MER

3. 2. Poly(A)-specific Ribonuclease (PARN) : Structural and Functional Studies of Poly(A) Recognition and Degradation

   Författare :Niklas Henriksson; Anders Virtanen; Marvin Wickens; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; Cell- och molekylärbiologi; Molecular Biotechnology; Molekylär bioteknik;

   Sammanfattning : Regulation of mRNA degradation is a powerful way for the cell to regulate gene expression. A critical step in eukaryotic mRNA degradation is the removal of the poly(A) tail at the 3'-end of the mRNA. LÄS MER

4. 3. Electrospray Ionization Mass Spectrometry for Determination of Noncovalent Interactions in Drug Discovery

   Författare :Kurt Benkestock; Johan Roeraade; Renato Zenobi; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; electrospray ionization; drug discovery; noncovalent interaction; complexes; human serum albumin HSA ; fatty acid binding protein FABP ; ribonuclease; ligand screening; Analytical chemistry; Analytisk kemi;

   Sammanfattning : Noncovalent interactions are involved in many biological processes in which biomolecules bind specifically and reversibly to a partner. Often, proteins do not have a biological activity without the presence of a partner, a ligand. LÄS MER

5. 4. Methods for the Synthesis of PET Tracers and NMR Studies of Ribonuclease A

   Författare :Linda Samuelsson; Bengt Långström; Sharon Stone-Elander; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; [11C]methyl iodide; Stille reaction; palladium; Ribonuclease A; osmolytes; PFG-NMR; Organisk kemi; Organic chemistry; Organisk kemi;

   Sammanfattning : This thesis contains two parts.In the first part, general and versatile palladium-mediated 11C-C bond forming reactions for use in the production of radiotracers for Positron Emission Tomography (PET) were explored. LÄS MER

7. 5. Allosteric Regulation of mRNA Metabolism : -Mechanisms of Cap-Dependent Regulation of Poly(A)-specific Ribonuclease (PARN)

   Författare :Per Nilsson; Anders Virtanen; Megerditch Kiledjian; Uppsala universitet; []
   Nyckelord :Cell and molecular biology; mRNA degradation; deadenylation; cap; poly A ; PARN; allosteric regulation; Cell- och molekylärbiologi;

   Sammanfattning : Degradation of mRNA is a highly regulated step important for proper gene expression. Degradation of eukaryotic mRNA is initiated by shortening of the 3’ end located poly(A) tail. Poly(A)-specific ribonuclease (PARN) is an oligomeric enzyme that degrades the poly(A) tail with high processivity. LÄS MER