Sökning: "binding specificities"

Visar resultat 11 - 15 av 62 avhandlingar innehållade orden binding specificities.

  1. 11. Deciphering the transcriptional regulation code in colorectal cancer genome

    Författare :Jian Yan; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Colorectal cancer (CRC) is the third most common cancer type to threaten life for both men and women in the developed world. The molecular mechanism of CRC is very complicated and involves changes in different categories of biological processes, among which a large number of mutations affecting either coding sequence of transcription factors (TFs) or their binding sites in genome are included. LÄS MER

  3. 12. Photochemical Ligation Techniques for Carbohydrate Biosensors and Protein Interaction Studies

    Författare :Oscar Norberg; Olof Ramström; Stéphane Vincent; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Carbohydrates; Biosensors; CuAAC; Photochemistry; Thiol-ene yne; Perfluorophenylazide PFPA ; Lectins; Molecular recognition; Quartz crystal microbalance QCM ; Surface plasmon resonance SPR ;

    Sammanfattning : This thesis concerns the development of surface ligation techniques for the preparation of carbohydrate biosensors. Several methodologies were developed based on efficient photochemical insertion reactions which quickly functionalize polymeric materials, with either carbohydrates or functional groups such as alkynes or alkenes. LÄS MER

  4. 13. Human Forkhead Genes

    Författare :Stefan Pierrou; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; transcription factor; FREAC; human forkhead homologues;

    Sammanfattning : The forkhead family constitutes a group of genes which encodes transcription factors that share a common DNA-binding domain, the forkhead domain. The first member to be discovered was the Drosophila melanogaster gene fork head. Members of this family have been found in a variety of species from bakers' yeast to man. LÄS MER

  5. 14. Engineering Antibody Specificity Using Combinatorial Antibody Libraries

    Författare :Jenny Boström; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; antibody Antigen Interactions; Dual Specific Antibodies; Herceptin; Human Epidermal Growth Factor Receptor 2; Thermodynamic Analysis; therapeutic Antibody Engineering; Synthetic Phagedisplayed Antibody Libraries; Vascular Endothelial Growth Factor; Bacterial Display; trastuzumab;

    Sammanfattning : The research presented in this thesis investigates antibody specificity, antibody-antigen and protein-protein interactions using combinatorial antibody libraries, phage-display and bacterial display technology. One goal was to develop a method to generate antibodies that bind two protein antigens with high affinity using a single-antigen binding site, and to investigate the molecular and thermodynamic requirements for antibody multispecificity. LÄS MER

  7. 15. Engineering alternative scaffold proteins for conditional targeting

    Författare :Malin Jönsson; Sophia Hober; Mark Howarth; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein Engineering; Conditional targeting; Calcium; Biotechnology; Bioteknologi;

    Sammanfattning : Engineering naturally occurring proteins enables us to customize affinity domains according to our specific needs, tailoring them to become an important tool in a wide array of applications limited merely by our creativity. One of nature’s ways to regulate protein activity is by creating a functional change through alteration of a protein’s tertiary structure upon interaction with metal ions. LÄS MER