Sökning: "therapeutic Antibody Engineering"

Visar resultat 1 - 5 av 27 avhandlingar innehållade orden therapeutic Antibody Engineering.

1. 1. Engineering Antibody Specificity Using Combinatorial Antibody Libraries

   Författare :Jenny Boström; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; antibody Antigen Interactions; Dual Specific Antibodies; Herceptin; Human Epidermal Growth Factor Receptor 2; Thermodynamic Analysis; therapeutic Antibody Engineering; Synthetic Phagedisplayed Antibody Libraries; Vascular Endothelial Growth Factor; Bacterial Display; trastuzumab;

   Sammanfattning : The research presented in this thesis investigates antibody specificity, antibody-antigen and protein-protein interactions using combinatorial antibody libraries, phage-display and bacterial display technology. One goal was to develop a method to generate antibodies that bind two protein antigens with high affinity using a single-antigen binding site, and to investigate the molecular and thermodynamic requirements for antibody multispecificity. LÄS MER

3. 2. Utilizing Solid Phase Cloning, Surface Display And Epitope Information for Antibody Generation and Characterization

   Författare :Francis Jingxin Hu; Johan Rockberg; John McCafferty; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody engineering; antibody affinity maturation; combinatorial protein engineering; epitope mapping; FACS; HER2; complement C5; Staphylococcal surface display; surface display.; Bioteknologi; Biotechnology;

   Sammanfattning : Antibodies have become indispensable tools in diagnostics, research and as therapeutics. There are several strategies to generate monoclonal antibodies (mAbs) in order to avoid the drawbacks of polyclonal antibodies (pAbs) for therapeutic use. LÄS MER

4. 3. Antibody Evolution and Repertoire Development

   Författare :Johan Lantto; Institutionen för immunteknologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Immunology; serology; transplantation; Immunologi; serologi; virus neutralization; trinucleotide repeat; insertion; immunoglobulin variable genes; human cytomegalovirus; glycoprotein B; deletion; antibody; antibody repertoire;

   Sammanfattning : Antibodies are key players of the immune system in higher vertebrates, which provide a defense against potentially lethal threats from the environment. Besides their importance in the immune defense, antibodies have a great potential as reagents in biological chemistry and diagnostics, and as therapeutic agents against both infectious diseases and cancer. LÄS MER

5. 4. Calcium-dependent Affinity Domains for the Purification of Antibodies and Antibody Fragments

   Författare :Julia Scheffel; Sophia Hober; Alois Jungbauer; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Antibodies; Antibody fragments; Biomanufacturing; Protein purification; Protein engineering; Protein A; ZCa; Calcium; Mild elution; Bioteknologi; Biotechnology;

   Sammanfattning : Antibodies are essential proteins in both our bodies and biotechnological research, and hold outstanding therapeutic value. The market for antibody-based therapeutics has grown exponentially during the last decades, owing to several advantages over small molecule drugs, such as fewer undesirable side effects associated with a higher target specificity. LÄS MER

7. 5. Cell line and protein engineering tools for production and characterization of biologics

   Författare :Anna-Luisa Volk; Johan Rockberg; Mathias Uhlen; Dickson Alan; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Pharmaceutical proteins; precision medicine; antibody engineering; epitope mapping; cell line development; split-GFP; Bioteknologi; Biotechnology;

   Sammanfattning : Our increasing understanding of disease mechanisms coupled with technological advances has facilitated the generation of pharmaceutical proteins, which are able to address yet unmet medical needs. Diseases that were fatal in the past can now be treated with novel biological medications improving and prolonging life for many patients. LÄS MER