Sökning: "amino acid mutagenesis"

Visar resultat 31 - 35 av 81 avhandlingar innehållade orden amino acid mutagenesis.

1. 31. Autoantigenic properties of the U1-70K protein

   Författare :Elisabet Welin Henriksson; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :U1-70K protein; ribonucleoproteins; autoantigens; autoantibodies; epitopemapping; connective tissue disease; species specificity; cross reactions; amino acidsequence; recombinant proteins; mutagenesis;

   Sammanfattning : Patients with the autoimmune diseases systemic lupus erythematosus and mixed connective tissue disease produce autoantibodies directed towards specific groups of cellular constituents in their own body. Two of the targeted antigens are denoted Sm and RNP, and consist of distinct collections of splicing associated RNA-protein complexes. LÄS MER

3. 32. Structure-function studies of plasminogen activator inhibitor-1

   Författare :Guang-Chao Sui; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :PAI-1; site-directed mutagenesis; structure and function; stability;

   Sammanfattning : Plasminogen activator inhibitor-I (PAI-1) is an important physiological inhibitor of tissue-type plasminogen activator (tPA) and urine-type plasminogen activator (uPA). High plasma levels of PAI-1 correlate to thrombotic disease, and low levels can be connected to a bleeding tendency. LÄS MER

4. 33. A study of allergens in the mite Lepidoglyphus destructor, using monoclonal antibodies and recombinant techniques

   Författare :Susanna Olsson; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :allergen; characterization; dust mite; IgE-reactivity; monoclonal antibody; recombinant allergen; site-directed mutagenesis;

   Sammanfattning : Mites are one of the most common causes of allergic reactions in many parts of the world. They can broadly be divided into two categories: house dust mites, primarily found in dwellings, and storage mites, primarily found in farming environments and grain storage facilities. Storage mites have now also been shown to exist in house dust. LÄS MER

5. 34. Enzymatic mechanisms in biotin synthesis : vitamin B6 catalysis and phosphoryl transfer

   Författare :Jenny Sandmark; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :X-ray crystallography; biotin biosynthesis; pyridoxal-5 -phosphate; aminotransferase; aluminium fluoride; S-adenosyl-L-methionine; 7; 8-diaminopelargonic acid synthase; dethiobiotin synthetase; biotin; phosphoryl transfer; amiclenomycin.;

   Sammanfattning : With the large number of pathogenic bacteria that have developed antibiotic resistance it is increasingly important to find new targets for antibiotics. Our interest in the biotin biosynthesis pathway originates from this drug design perspective. All organisms require biotin, but it is only synthesised in bacteria, in plants and in some fungi. LÄS MER

7. 35. Short-chain dehydrogenases/reductases : structure, function and motifs of hydroxysteroid dehydrogenases

   Författare :Charlotta Filling; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Short-chain dehydrogenases reductases; site-directed mutagenesis; steady-state kinetics; X-ray structure; enzyme function; steroid; motif; reaction mechanism; 3 beta 17 beta-hydroxysteroid dehydrogenase; 17 beta-hydroxysteroid dehydrogenase; short chain hydroxyacyl coenzyme A dehydrogenase;

   Sammanfattning : Short-chain dehydrogenases/reductases (SDR) constitute a superfamily of proteins catalysing reactions of a wide range of substrates in all life forms. During the past years, a large number of new proteins belonging to this superfamily has been detected. They are important in e.g. LÄS MER