Sökning: "3 beta 17 beta-hydroxysteroid dehydrogenase"

Hittade 3 avhandlingar innehållade orden 3 beta 17 beta-hydroxysteroid dehydrogenase.

  1. 1. X-ray structure analysis of short-chain dehydrogenases/reductases

    Författare :Jordi Benach-Andreu; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :crystal structure; short-chain dehydrogenases reductases; Drosophila alcohol dehydrogenase; catalytic reaction mechanism; Comamonas testosteroni 3[beta] 17[beta]hydroxysteroid dehydrogenase;

    Sammanfattning : X-ray crystallography and site-directed mutagenesis were used to better understand the structure/function relationships in the family of short-chain dehydrogenases/reductases (SDR). This group of enzymes constitutes a protein family with at least 60 members with highly diverse functions in pro- and eukaryotes. LÄS MER

  3. 2. Short-chain dehydrogenases/reductases : structure, function and motifs of hydroxysteroid dehydrogenases

    Författare :Charlotta Filling; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Short-chain dehydrogenases reductases; site-directed mutagenesis; steady-state kinetics; X-ray structure; enzyme function; steroid; motif; reaction mechanism; 3 beta 17 beta-hydroxysteroid dehydrogenase; 17 beta-hydroxysteroid dehydrogenase; short chain hydroxyacyl coenzyme A dehydrogenase;

    Sammanfattning : Short-chain dehydrogenases/reductases (SDR) constitute a superfamily of proteins catalysing reactions of a wide range of substrates in all life forms. During the past years, a large number of new proteins belonging to this superfamily has been detected. They are important in e.g. LÄS MER

  4. 3. Functional genomics at the interface of protein expression and biophysical analysis

    Författare :Xiaoqiu Wu; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Protein characterization is central to any functional genomics effort, hence protein expression and purification play fundamental roles in these initiatives. In this study strategies for protein expression in E. coli were analysed, and several parameters were investigated respect to high-level expression of functional proteins. LÄS MER