Sökning: "Unnatural Amino Acids"

Visar resultat 1 - 5 av 12 avhandlingar innehållade orden Unnatural Amino Acids.

1. 1. In Vitro Kinetics of Ribosomal Incorporation of Unnatural Amino Acids

   Författare :Jinfan Wang; Anthony Forster; Jason Chin; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; ribosome; protein synthesis; unnatural amino acids; kinetics; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

   Sammanfattning : Ribosomal incorporation of unnatural amino acids (AAs) into peptides or proteins has found broad applications in studying translation mechanism, discovering potential therapeutics, and probing protein structure and function. However, such applications are generally limited by the low incorporation efficiencies of the unnatural AAs. LÄS MER

3. 2. Rate and Accuracy of Bacterial Protein Synthesis with Natural and Unnatural Amino Acids

   Författare :Ka-Weng Ieong; Måns Ehrenberg; Simpson Joseph; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ribosome; protein synthesis; translation; efficiency-accuracy trade-off; kinetics; unnatural amino acids; Molecular Biology; Molekylärbiologi;

   Sammanfattning : This thesis addresses different questions regarding the rate, efficiency, and accuracy of peptide bond formation with natural as well as unnatural amino acids: Which step is rate-limiting during peptide bond formation? How does the accuracy vary with different transfer RNAs (tRNAs) and codons and how is it relevant to the living cells? Does proofreading selection of codon reading occur in a single- or multi-step manner as theoretically suggested? How does the E. coli translation system discriminate unnatural amino acids? Based on that, how to improve the incorporation efficiencies of unnatural amino acids?Based on the study on pH dependence of peptide bond formation, we show that the rate of the chemistry of peptidyl transfer to aminoacyl-tRNA (AA-tRNA) Gly-tRNAGly or Pro-tRNAPro limits the rate of peptide bond formation at physiological pH 7. LÄS MER

4. 3. Synthesis of Non-Natural Aromatic Amino Acids Derivatives Solvent-free reactions-Versatile building blocks-Galectin inhibitors

   Författare :Erik Tullberg; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Organisk kemi; Organic chemistry; Naturvetenskap; Natural science; Multivalent galectin inhibitors; Multifunctional molecules; Amino Acids; Solvent-free reactions;

   Sammanfattning : This thesis deals with three topics concerning the synthesis of non-natural also known as unnatural amino acid derivatives. In the first, solvent-free reaction conditions for the Heck reaction were developed that were superior to previous conditions because the reaction time could be reduced and the work-up procedure simplified. LÄS MER

5. 4. Ribosomal Peptidyl Transfer with Natural and Unnatural Amino Acids

   Författare :Ka-Weng Ieong; Uppsala universitet; []
   Nyckelord :;

   Sammanfattning : .... LÄS MER

7. 5. Exotic Ribosomal Enzymology

   Författare :Josefine Liljeruhm; Anthony C. Forster; Christophe Danelon; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; rRNA modification; Methyltransferase; RlmM; RlmJ; D-amino acid; Unnatural amino acid; Chromoprotein; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

   Sammanfattning : This thesis clarifies intriguing enzymology of the ribosome, the multiRNA/multiprotein complex that catalyzes protein synthesis (translation). The large ribosomal RNAs (23S and 16S rRNAs in E. coli) are post-transcriptionally modified by many specific modification enzymes, yet the functions of the modifications remain enigmatic. LÄS MER