Sökning: "fluorescence resonance energy transfer FRET"

Visar resultat 1 - 5 av 25 avhandlingar innehållade orden fluorescence resonance energy transfer FRET.

  1. 1. Electronic Energy Transfer within Asymmetric Pairs of Fluorophores: Partial Donor-Donor Energy Migration (PDDEM)

    Författare :Stanislav Kalinin; Lennart B.-Å. Johansson; Helge Lemmetyinen; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Physical chemistry; fluorescence resonance energy transfer FRET ; donor-donor energy migration DDEM ; homotransfer; fluorescence relaxation; lifetimes; time-resolved fluorescence anisotropy; time-correlated single photon counting; distance measurements; protein structure; Fysikalisk kemi; Physical chemistry; Fysikalisk kemi; Physical Chemistry; fysikalisk kemi;

    Sammanfattning : A kinetic model of electronic energy migration within pairs of photophysically non-identical fluorophores has been developed. The model applies to fluorescent groups that exhibit different photophysical and spectral properties when attached to different positions in a macromolecule. LÄS MER

  3. 2. Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics

    Författare :Joachim Piguet; Horst Vogel; Ruud Hovius; Jerker Widengren; Antoine Triller; Switzerland Ecole Polytechnique Fédérale de Lausanne (EPFL); []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; fluorescence; microscopy; plasma membrane; membrane proteins; membrane proteins diffusion; single-molecule imaging; single-molecule tracking; pentameric ligand-gated ion channels; nicotinic acetylcholine receptor; serotonin receptor; synapse; neuromuscular junction; post-synaptic scaffold; rapsyn; myopathies; fluorescent proteins; Förster resonance energy transfer; FRET imaging; protein-protein interactions; protein trafficking; photo-activatable proteins; Biological Physics; Biologisk fysik;

    Sammanfattning : Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotinic acetylcholine is densely packed under the motor nerve termini. LÄS MER

  4. 3. Förster Resonance Energy Transfer - from single molecule spectroscopy to imaging

    Författare :Evangelos Sisamakis; Jerker Widengren; Paul R Selvin; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; FRET; FCS; single-molecule biophysics;

    Sammanfattning : During the last fifteen years several methods have been developed for probing biomolecules (DNA, RNA, proteins) one at a time. Among these methods fluorescence spectroscopy and in particular its many implementations for monitoring Förster Resonance Energy Transfer (FRET), have attracted much interest. LÄS MER

  5. 4. DNA and RNA base analogue FRET - from fluorophore design to biochemical applications

    Författare :Moa Sandberg Wranne; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; adenine analogue; Fluorescent base analogue; FRET; thymine analogue; netropsin; fluorescence; DNA; RNA; Z-RNA;

    Sammanfattning : This thesis focuses on the development and use of fluorescent base analogues (FBAs). They are important tools in research concerning nucleic acids structure, dynamics and interactions. LÄS MER

  7. 5. DNA-Controlled Lipid-Membrane Fusion

    Författare :Lisa Simonsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; exocytosis; vesicles; DNA; FRET; fluorescence resonance energy transfer; model system; total internal reflection fluorescence microscopy; TIRF microscopy; self-assembly; site-specificity; diffusion; biomembranes; lipid bilayer; membrane fusion;

    Sammanfattning : Membrane fusion is essential for nerve-cell communication, for protein transport between cell organelles and the cell-membrane and for enabling the merger between virus and host membranes during virus infection. We have demonstrated that short DNA oligonucleotides, membrane-attached via CH in an orientation that mimics the overall zipperlike architecture of fusion-inducing proteins, induce fusion of both suspended vesicles and vesicles site-specifically tethered to SLBs. LÄS MER