Sökning: "bound target protein"

Visar resultat 1 - 5 av 149 avhandlingar innehållade orden bound target protein.

1. 1. Molecular principles of protein stability and protein-protein interactions

   Författare :Christofer Lendel; Per-Åke Nygren; Christopher M. Dobson; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; binding thermodynamics; induced fit; molten globule; NMR spectroscopy; protein engineering; protein-protein interactions; protein stability; protein structure.; Structural biochemistry; Strukturbiokemi;

   Sammanfattning : Proteins with highly specific binding properties constitute the basis for many important applications in biotechnology and medicine. Immunoglobulins have so far been the obvious choice but recent advances in protein engineering have provided several novel constructs that indeed challenge antibodies. LÄS MER

3. 2. Structure determination and thermodynamic stabilization of an engineered protein-protein complex

   Författare :Elisabet Wahlberg; Per-Åke Nygren; Bengt-Harald Jonsson; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; protein structure; coupled folding; NMR spectroscopy; protein stability; protein-protein interactions; binding thermodynamics; isothermal titration calorimetry; protein engineering; Structural biochemistry; Strukturbiokemi;

   Sammanfattning : The interaction between two 6 kDa proteins has been investigated. The studied complex of micromolar affinity (Kd) consists of the Z domain derived from staphylococcal protein A and the related protein ZSPA-1, belonging to a group of binding proteins denoted affibody molecules generated via combinatorial engineering of the Z domain. LÄS MER

4. 3. Amyloid Beta – Biotherapy Target and Biotechnological Tool

   Författare :Sebastian W. Meister; Stefan Ståhl; John Löfblom; Maarten Merkx; KTH; []
   Nyckelord :Alzheimer’s disease; amyloid beta; blood-brain barrier; green-fluorescent protein; intracellular assays; proteases; protease substrate specificity; protein aggregation; protein engineering.; Bioteknologi; Biotechnology;

   Sammanfattning : Alzheimer’s disease is a progressive neurodegenerative disease characterized primarily by memory impairment and cognitive decline. As of 2020, an estimated 50 million people suffer from Alzheimer’s disease or related dementia and no disease-modifying treatment options are yet approved for clinical practice. LÄS MER

5. 4. Protein Microarray Chips

   Författare :Goran Klenkar; Bo Liedberg; Brian MacCraith; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Microarray; biosensor; biomolecular interaction; narcotics detection; imaging ellipsometry and surface plasmon resonance; Molecular biophysics; Molekylär biofysik;

   Sammanfattning : Livet tas för givet av de flesta. Det finns däremot många som ägnar stora delar av sitt liv för att försöka lösa dess mysterier. En del av lösningen ligger i att förstå hur alla molekyler är sammanlänkade i det gigantiska nätverk som definierar den levande organismen. LÄS MER

7. 5. Protein production, characterization and structure determination in structural genomics

   Författare :Esmeralda A. Woestenenk; Torleif Härd; Göran Karlsson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biology; protein production; protein solubility; fusion tags; nuclear magnetic resonance; structure determination; Biologi; Biology; Biologi;

   Sammanfattning : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy. The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. LÄS MER