Sökning: "DNA-DNA hybridisation"

Hittade 4 avhandlingar innehållade orden DNA-DNA hybridisation.

  1. 1. A thermostable alkaline protease from a new alkaliphilic Nesterenkonia sp

    Författare :Shahrzad Bakhtiar; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; substrate specificity; crystallization; Biotechnology; Bioteknik; DNA-DNA hybridisation; 16S rDNA analysis; phenotypic characterisation; circular dichroism; differential scanning calorimetry; Nesterenkonia abyssinica; stability; calcium-independent; alkaline protease; alkaliphilic;

    Sammanfattning : This thesis concerns a novel alkaline protease produced by an extremophilic microbial specie, designated as AL20, isolated from a feather sample collected at the shore of the alkaline soda lake Abjata in the Ethiopian Rift Valley. The isolate is a heterotrophic, alkaliphilic, halotolerant gram-positive, strictly aerobic, non-motile, non-spore forming bacterium. LÄS MER

  3. 2. The female genital tract microbiota: composition, relation to innate immune factors, and effects of contraceptives

    Författare :Natalia Nikolaitchouk; Göteborgs universitet; []
    Nyckelord :bacterial vaginosis; checkerboard DNA-DNA hybridisation; cytokines; SLPI; Lactobacillus coleohominis; Lactobacillus iners; Actinomyces urogenitalis;

    Sammanfattning : Abnormal vaginal microbiota, as in bacterial vaginosis (BV), is associated with increased risk of obstetrical and gynaecologic complications and acquisition of sexually transmitted diseases. However, very little is known about the pathogenesis of BV. In BV, the normal vaginal Lactobacillus-dominated biota (LDB) is replaced by anaerobic bacteria. LÄS MER

  4. 3. The development of synthetic gene delivery systems

    Författare :Lars J Brandén; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :gene transfer; synthetic vector; gene therapy; Bioplex; PNA; NLS;

    Sammanfattning : This thesis describes a new platform technology. The technique is referred to as Bioplex (biological complex) in analogy with the nomenclature for other transfection reagents, since we attach biological functions to a nucleic acid complex via sequence-specific hybridisation. LÄS MER

  5. 4. Presence and viability of enterotoxigenic Escherichia coli (ETEC) in aquatic environments

    Författare :Åsa Lothigius; Göteborgs universitet; []
    Nyckelord :Enterotoxigenic Escherichia coli; ETEC; real-time PCR; aquatic environments; VBNC; resuscitation; infant mouse model;

    Sammanfattning : Enterotoxigenic Escherichia coli (ETEC) is one of the major causes of diarrhoea among children in developing countries and in travelers to these regions. The bacteria are spread via contaminated water and food, and surface and drinking water in developing countries has been found to harbor these organisms. LÄS MER