Sökning: "Antibody microarrays"
Visar resultat 16 - 20 av 32 avhandlingar innehållade orden Antibody microarrays.
16. On the application and validation of multiplexed affinity assays
Sammanfattning : Proteins are essential macromolecules that carry out complex functions in human cells, tissues, and organs. They regulate a diverse set of biological processes and protect against pathogens. However, dysregulation or malformation of proteins can cause disease. LÄS MER
17. Novel methods for improving rapid paper-based protein assays with gold nanoparticle detection
Sammanfattning : This thesis describes methods for improving sensitivity in rapid singleplex and multiplex microarray assays. The assays utilize the optical characteristics of colloidal gold nanoparticles for the colorimetric detection of proteins.Multiplexed detection in sandwich immunoassays is limited by cross-reactivity between different detection antibodies. LÄS MER
18. Advances and Applications of Antibody Arrays - Proteomic Profiling of Pancreatic Disease
Sammanfattning : Recombinant antibody microarrays have advanced into indispensable tools for large-scale, high-throughput multiplexed serum proteomics. This thesis, based upon five original papers, deals with the development of an in-house designed antibody microarray platform, and its applications for serum profiling of pancreatic disease. LÄS MER
19. Biomarker Discovery in Cutaneous Malignant Melanoma : A Study Based on Tissue Microarrays and Immunohistochemistry
Sammanfattning : The incidence of cutaneous malignant melanoma has increased dramatically in Caucasians the last few decades, an increase that is partly explained by altered sun exposure habits. For the individual patient, with a localized disease, the tumor thickness of the excised lesion is the most important prognostic factor. LÄS MER
20. Molecular Portraits of Cancer : Discovery of Biomarker Signatures using Affinity Proteomics
Sammanfattning : The use of antibodies as capture molecules in assays is a common practice. By either printing monoclonal single chain fragment variables (scFvs) on a solid support – recombinant antibody microarray – or attaching them to magnetic beads – Global Proteome Survey (GPS) – we can specifically capture and measure target proteins of interest. LÄS MER