Sökning: "whole genome shotgun"

Visar resultat 1 - 5 av 6 avhandlingar innehållade orden whole genome shotgun.

  1. 1. Solving repeat problems in shotgun sequencing

    Författare :Erik Arner; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Shotgun sequencing; repeated DNA; genomics; whole genome shotgun; hierarchical shotgun; mate pairs; DNP; bioinformatics;

    Sammanfattning : Shotgun sequencing is the most powerful strategy for large scale sequencing. Two main approaches exist: clone-by-clone and whole genome shotgun (WGS). In the clone-by-clone strategy, overlapping clones are amplified and then sheared in a random fashion. LÄS MER

  3. 2. Massively parallel analysis of cells and nucleic acids

    Författare :Julia Sandberg; Joakim Lundeberg; Nader Pourmand; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Massively parallel sequencing; 454; Illumina; multiplex amplification; whole genome amplification; single cell; polyguanine; flow-cytometry;

    Sammanfattning : Recent proceedings in biotechnology have enabled completely new avenues in life science research to be explored. By allowing increased parallelization an ever-increasing complexity of cell samples or experiments can be investigated in shorter time and at a lower cost. LÄS MER

  4. 3. Molecular epidemiologic studies on helicobacter pylori infection and stomach cancer risk

    Författare :Zongli Zheng; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Helicobacter pylori;

    Sammanfattning : Helicobacter pylori (H. pylori) infection increases stomach cancer risk. The aim of this thesis was to study genetic susceptibility from the host and to develop molecular methods for future characterization of bacterial virulence factors in longitudinal cohorts. LÄS MER

  5. 4. Mass spectrometry based proteomics : data analysis and applications

    Författare :Yafeng Zhu; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Mass spectrometry (MS) based proteomics has become a widely used high throughput method to investigate protein expression and functional regulation. From being able to study only dozens of proteins, state-of-art MS proteomic techniques are now able to identify and quantify ten thousand proteins. LÄS MER

  7. 5. On the origin of geometrid moths: the phylogenetic relationships and taxonomy

    Författare :Leidys Murillo; Biodiversitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : Geometrid moths represent one of the largest radiations of Lepidoptera. Phylogenetic analyses based on morphological and molecular data supports the monophyly of this group of moths. However, the subfamilial and tribal compositions of major groups are still unresolved, leading to unstable classifications. LÄS MER