Sökning: "thermostable"

Visar resultat 11 - 15 av 38 avhandlingar innehållade ordet thermostable.

  1. 11. Biochemical and structural characterization of two thermostable carbohydrate-active enzymes with potential in biotechnological applications

    Författare :Noor Hassan; Christina Divne; Torbjörn Gräslund; Henrik Hansson; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : .... LÄS MER

  3. 12. Xylan degradation by the thermophilic bacterium Rhodothermus marinus. Characterization and function of a thermostable xylanase

    Författare :Eva Nordberg Karlsson; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; fed-batch cultivation; kraft pulp; bleaching; glycosyl hydrolase family 10; recombinant xylanase; polysaccharide binding domain; Biotechnology; Bioteknik;

    Sammanfattning : A gene encoding a multidomain xylanase from the thermophilic bacterium Rhodothermus marinus has been cloned, sequenced and produced in Escherichia coli . The gene product was found to be a 109 kDa protein consisting of five domains. LÄS MER

  4. 13. RECOMBINANT PEROXIDASES AND XYLANASES: I. Cloning and production of a peroxidase from horseradish II. Characterisation of functional domains of thermostable xylanases from Rhodothermus marinus

    Författare :Eva Bartonek Roxå; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; amorphous cellulose; xylan; CBD; carbohydrate binding domains; insect cells; Baculovirus; catalytically active; recombinant horseradish peroxidase; HRP-n; PASC; beta-glucan; Biotechnology; Bioteknik;

    Sammanfattning : Part I: Plant peroxidases are useful in many areas of application e.g. as tracer enzymes for clinical applications and in biosensors as well as for industrial purposes like in waste water treatment and bleaching processes. A gene encoding a new, neutral horseradish peroxidase isoenzyme, HRP-n, was isolated. LÄS MER

  5. 14. Bench-Scale Production of Heterologous Proteins from Extremophiles- Escherichia coli and Pichia pastoris based expression systems

    Författare :Santosh Ramchuran; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Glycoside hydrolase; Heterologous protein; High cell density cultivation Expression systems; Bioteknik; Biotechnology; Fed-batch;

    Sammanfattning : Over the past few years considerable research attention has been assigned to extremophiles as sources of extremozymes due to their applicability in industrial processes, and the development of eco-friendly technologies. The establishment of efficient production strategies for heterologous proteins is an empirical process requiring broad background knowledge on available expression systems together with their major advantages and shortcomings. LÄS MER

  7. 15. On the stability of proteins from hyperthermophiles : a structural and thermodynamic study

    Författare :Stefan Knapp; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Protein stability; hyperthermophiles; X-ray crystallography; glutamate dehydrogenase; Sso7d.;

    Sammanfattning : In this study the structural and thermodynamic basis of the thermal stability of proteins has been studied using proteins from hyperthermophilic organisms as model systems. A new type of chaperonin (TF55) has been isolated from the hyperthermophilic archaeon Sulfolobus solfataricus consisting of two different subunits named TF55-alpha and TF55-beta. LÄS MER