Sökning: "serum-free medium"

Visar resultat 1 - 5 av 21 avhandlingar innehållade orden serum-free medium.

  1. 1. Impact of autocrine factors on physiology and productivity in Trichoplusia ni serum-free cultures

    Författare :Ulrika Eriksson; Lena Häggström; Per Berglund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Trichoplusia ni; High Five; insect cells; BEVS; serum-free medium; yeast extract; conditioned medium; autocrine regulation of proliferation; growth factors; cell cycle; metalloproteinase; specific productivity; Bioteknik; Bioengineering; Bioteknik;

    Sammanfattning : The aim of this study was to increase the understanding of the mechanisms regulating cell proliferation and recombinant protein production in serum-free cultures of Trichoplusia ni (T. ni) insect cells.Conditioned medium (CM) was shown to contain both stimulatory and inhibitory factors (CM factors) influencing cell growth. LÄS MER

  3. 2. Regulation of productivity in Trichoplusia ni and Spodoptera frugiperda Sf9 serum-free cultures

    Författare :Karin Calles; Lena Häggström; Gunnel Dalhammar; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Insect cells; BEVS; Spodoptera frugiperda; Sf9; Trichoplusia ni; BTI-Tn-5B1-4; specific productivity; conditioned medium; conditioned medium factors; cell cycle; synchronization; G2 M; Bioteknik; Bioengineering; Bioteknik;

    Sammanfattning : The aim of this work has been to characterize the effects of conditioned medium (CM) on insect cell productivity and physiology in order to get a better understanding about the mechanisms that regulate productivity in serum-free media. Two cell lines have been investigated, Spodoptera frugiperda (Sf9) and Trichoplusia ni (T. ni, BTI-Tn-5B1-4). LÄS MER

  4. 3. Physiological effects of conditioned medium and passage number on Spodoptera frugiperda Sf9 serum free cultures

    Författare :Ingrid Svensson; Lena Häggström; Mats Martinelle; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Spodoptera frugiperda Sf9; insect cell cultures; conditioned medium; cell cycle phase dynamics; proteolytic activity; antibacterial activity; recombinant protein production; Bioteknik; Bioengineering; Bioteknik;

    Sammanfattning : The aim of this study was to better understand the role of conditioned medium (CM) in Spodoptera frugiperda Sf9 insect cell proliferation and recombinant protein production using the baculovirus expression system. CM was found to stimulate cell proliferation. LÄS MER

  5. 4. The Germinal Centre reaction - Genetic and proteomic analysis of factors important for survival and growth of B lymphocytes

    Författare :Linda Zander; Göteborgs universitet; []
    Nyckelord :B lymphocyte; germinal centre; transformation; serum-free; MHC class II; bare lymphocyte syndrome; extracellular proteins; DNA repair; Polymerase ?;

    Sammanfattning : During an immune response the B lymphocytes main function is to produce antibodies in the specific defence against the pathogen. When naïve B lymphocytes become activated by binding of an antigen, the cells differentiate to become antibody producing plasma cells. LÄS MER

  7. 5. Physiology and productivity of serum-free Spodoptera frugiperda Sf9 insect cell cultures

    Författare :Eva Lindskog; Lena Häggström; Spiros N. Agathos; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Animalcellteknologi; Bioengineering; Bioteknik;

    Sammanfattning : The objective of this study was to investigate the mechanisms and factors controlling growth and proliferation in serum-free Spodoptera frugiperda Sf9 cultures as well as the implications of these factors for protein production in the baculovirus expression system. The physiology of recently thawed, low passage (Lp) Sf9 cultures, were compared to high passage (Hp) cultures at p>100. LÄS MER