Sökning: "cell cycle phase dynamics"

Visar resultat 1 - 5 av 16 avhandlingar innehållade orden cell cycle phase dynamics.

1. 1. Statistical Modelling of Cell Cycle Dynamics Analysis and Interpretation of BrdUrd DNA FCM-Derived Data

   Författare :Sara Larsson Lönn; Matematisk statistik; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; G2 phase duration; DNA replication; flow cytometry; Natural science; Naturvetenskap; S phase duration; DNA distribution; branching processes; cell cycle kinetics;

   Sammanfattning : It it of great importance to increase the knowledge of various cell cycle kinetic parameters and the objective of this thesis is to use stochastic models to interpret Bromodeoxyuridine (BrdUrd) DNA Flow Cytometry (FCM) derived data in order to estimate such parameters. The cell cycle is the process of growth and division that is essential for an organism to increase in size. LÄS MER

3. 2. Physiological effects of conditioned medium and passage number on Spodoptera frugiperda Sf9 serum free cultures

   Författare :Ingrid Svensson; Lena Häggström; Mats Martinelle; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Spodoptera frugiperda Sf9; insect cell cultures; conditioned medium; cell cycle phase dynamics; proteolytic activity; antibacterial activity; recombinant protein production; Bioteknik; Bioengineering; Bioteknik;

   Sammanfattning : The aim of this study was to better understand the role of conditioned medium (CM) in Spodoptera frugiperda Sf9 insect cell proliferation and recombinant protein production using the baculovirus expression system. CM was found to stimulate cell proliferation. LÄS MER

4. 3. A molecular chaperone that governs membrane contact sites and lipid metabolism

   Författare :Sergi Tosal Castaño; Sabrina Büttner; W. Mike Henne; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Yeast; nucleus-vacuole junctions; proteostasis; unfolded protein response; fatty acid elongation; molekylär biovetenskap; Molecular Bioscience;

   Sammanfattning : Transmembrane proteins represent almost one third of the total cellular proteome, and the majority of them requires translocation into the endoplasmic reticulum (ER) to be folded, sorted and transported to their final subcellular destination. High translational rates during exponential cell growth result in a massive influx of immature proteins into the ER, requiring rapid and accurate folding. LÄS MER

5. 4. Impact of autocrine factors on physiology and productivity in Trichoplusia ni serum-free cultures

   Författare :Ulrika Eriksson; Lena Häggström; Per Berglund; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Trichoplusia ni; High Five; insect cells; BEVS; serum-free medium; yeast extract; conditioned medium; autocrine regulation of proliferation; growth factors; cell cycle; metalloproteinase; specific productivity; Bioteknik; Bioengineering; Bioteknik;

   Sammanfattning : The aim of this study was to increase the understanding of the mechanisms regulating cell proliferation and recombinant protein production in serum-free cultures of Trichoplusia ni (T. ni) insect cells.Conditioned medium (CM) was shown to contain both stimulatory and inhibitory factors (CM factors) influencing cell growth. LÄS MER

7. 5. Functional roles of protein phase separated assemblies in cellular stress response and proteinopathies

   Författare :Michelle Lindström; Göteborgs universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; phase separation; LLPS; stress granules; Lsm7; FUS; Ccr4;

   Sammanfattning : For a long time, pertinent biological conundrums, such as the organization and compartmentalization of the cytoplasm and nucleoplasm, perplexed the scientific community. The organization of the complex biochemistry in an accurate space and time manner could not be explained without the enclosure of a membrane. LÄS MER