Sökning: "proteomik"

Visar resultat 31 - 35 av 78 avhandlingar innehållade ordet proteomik.

  1. 31. Integration of RNA and protein expression profiles to study human cells

    Författare :Frida Danielsson; Emma Lundberg; Mikael Huss; Mathias Uhlén; Christine Vogel; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; RNA-seq; Transcriptomics; Proteomics; Malignant transformation; Cancer; Functional enrichment; Bioteknologi; Biotechnology; Medical Technology; Medicinsk teknologi;

    Sammanfattning : Cellular life is highly complex. In order to expand our understanding of the workings of human cells, in particular in the context of health and disease, detailed knowledge about the underlying molecular systems is needed. LÄS MER

  3. 32. Targeted proteomics methods for protein quantification of human cells, tissues and blood

    Författare :Fredrik Edfors; Mathias Uhlén; Albert Heck; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; proteomics; mass spectrometry; protein quantification; stable isotope standard; parallel reaction monitoring; immuno-enrichment; Bioteknologi; Biotechnology;

    Sammanfattning : The common concept in this thesis was to adapt and develop quantitative mass spectrometric assays focusing on reagents originating from the Human Protein Atlas project to quantify proteins in human cell lines, tissues and blood. The work is based around stable isotope labeled protein fragment standards that each represent a small part of a human protein-coding gene. LÄS MER

  4. 33. Bioparticle Manipulation using Acoustophoresis and Inertial Microfluidics

    Författare :Muhammad Asim Faridi; Aman Russom; Martin Wiklund; Jonas Tegenfeldt; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biotechnology; Bioteknologi;

    Sammanfattning : Despite the many promising advances made in microfluidics, sample preparation remains the single largest challenge and bottleneck in the field of miniaturised diagnostics. This thesis is focused on the development of sample preparation methods using active and passive particle manipulation techniques for point of care diagnostic applications. LÄS MER

  5. 34. Characterization of antibody specificity using peptide array technologies

    Författare :Björn Forsström; Mathias Uhlén; Markus Templin; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody; Epitope mapping; Peptide array; Suspension bead array; Antigen; Specificity; Cross-reactivity; Immunization; Immunogenicity; Bioteknologi; Biotechnology;

    Sammanfattning : Antibodies play an important role in the natural immune response to invading pathogens. The strong and specific binding to their antigens also make them indispensable tools for research, diagnostics and therapy. LÄS MER

  7. 35. Utilizing Solid Phase Cloning, Surface Display And Epitope Information for Antibody Generation and Characterization

    Författare :Francis Jingxin Hu; Johan Rockberg; John McCafferty; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody engineering; antibody affinity maturation; combinatorial protein engineering; epitope mapping; FACS; HER2; complement C5; Staphylococcal surface display; surface display.; Bioteknologi; Biotechnology;

    Sammanfattning : Antibodies have become indispensable tools in diagnostics, research and as therapeutics. There are several strategies to generate monoclonal antibodies (mAbs) in order to avoid the drawbacks of polyclonal antibodies (pAbs) for therapeutic use. LÄS MER