Sökning: "periplasm"

Visar resultat 6 - 10 av 24 avhandlingar innehållade ordet periplasm.

1. 6. Growth rate control of periplasmic product retention in Escherichia coli

   Författare :Emma Bäcklund; Gen Larsson; Stefan Schmidt; Amelie Eriksson Karlström; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Escherichia coli; fed-batch; outer membrane proteins; recombinant proteins; specific growth rate; periplasmic retention; phosphotransferase system; high cell density cultivation; acetate formation; Bioengineering; Bioteknik;

   Sammanfattning : The recombinant product is secreted to the periplasm in many processes where E. coli is used as host. One drawback with secretion is the undesired leakage of the periplasmic products to the medium. The aim of this work was to find strategies to influence the periplasmic retention of recombinant products. LÄS MER

3. 7. Methodology for high-throughput production of soluble recombinant proteins in Escherichia coli

   Författare :Katrin Markland; Gen Larsson; Pär Nordlund; Jenny Ottosson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; high-throughput methodology; recombinant protein production; solubility; productivity; quality; cultivation technology; parallel reactors; Biochemistry; Biokemi;

   Sammanfattning : The aim of this work was to investigate and determine central parameters that can be used to control and increase the solubility, quality and productivity of recombinant proteins. These central parameters should be applicable under the constraints of high-throughput protein production in Escherichia coli. LÄS MER

4. 8. Protein production in the E. coli cell envelope

   Författare :Thomas Baumgarten; Jan-Willem de Gier; Dirk-Jan Scheffers; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; E. coli; protein biogenesis; recombinant proteins; membrane proteins; secretory proteins; protein displays; outer membrane vesicles; Biochemistry; biokemi;

   Sammanfattning : Proteins fulfil essential functions in every cell and malfunctioning proteins are often the cause of diseases. On the other hand, proteins like antibody fragments or hormones can be used to treat diseases. LÄS MER

5. 9. Protein targeting, translocation and insertion in Escherichia coli : Proteomic analysis of substrate-pathway relationships

   Författare :Louise Baars; Jan-Willem de Gier; Jan-Maarten van Dijl; Stockholms universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein biogenesis; targeting; translocation; insertion; SecB; SecE; E. coli; Biochemistry; Biokemi; biokemi; Biochemistry;

   Sammanfattning : Approximately 10% of the open reading frames in the genome of the Gram-negative bacterium E. coli encodes secretory proteins, and 20% encodes integral inner membrane proteins (IMPs). LÄS MER

7. 10. Optimizing membrane and secretory protein production in Gram-negative bacteria

   Författare :Anna Hjelm; Jan-Willem de Gier; Alex Toftgaard Nielsen; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; biokemi;

   Sammanfattning : Proteins fulfil a wide variety of essential functions in the cell. Recombinant protein production in the Gram-negative bacterium Escherichia coli (E. coli) facilitates structural and functional studies of proteins and it has been instrumental in biotechnology for the manufacturing of e.g. LÄS MER