Sökning: "gel electrophoresis"

Visar resultat 11 - 15 av 232 avhandlingar innehållade orden gel electrophoresis.

1. 11. Formation and Characterization of DNA Concatemers for Single Molecule Mechanism Studies

   Författare :Lu Sun; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; concatemers; hybridization; DNA; gel electrophoresis; self-assembly; constructs; nanotechnology;

   Sammanfattning : Well known as genetic information carrier, DNA however, in the past three decade experienced a revolution to become a fashionable fabrication material in nanotechnology as a consequence of the high predictability and accuracy of base-paring recognition. Although DNA can be constructed in many fancy 2D and 3D structures, this thesis work focused on constructing one-dimension linear DNA concatemers in order to study sequence specific DNA-ligand binding at the single-molecule level. LÄS MER

3. 12. Concatemerization of Synthetic Oligonucleotides

   Författare :Lu Sun; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; gel electrophoresis; DNA-binding; QCM-D; DNA concatemer; hybridization; DNA formation; self-assembly;

   Sammanfattning : DNA nanotechnology has become an important research field because of its advantages in high predictability and accuracy of base-paring recognition. For example, the DNA concatemer, one of the simplest DNA constructs in shape, has been used to enhance signals in biosensing. LÄS MER

4. 13. In vitro and in vivo studies of salivary films at solid/liquid interfaces

   Författare :Ida Svendsen; Malmö högskola; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; saliva; proteins; adsorption; ellipsometry; gel electrophoresis;

   Sammanfattning : A conditioning film, the pellicle, of which many salivary proteins areimportant constituents, covers the surfaces present in the mouth. The pellicleforms in a selective adsorption process, and it has protective and lubricatingfunctions as well as an influence on the adherence of oral microbes thatultimately leads to the development of dental plaque. LÄS MER

5. 14. Parallel target selection by trinucleotide threading

   Författare :Pawel Zajac; Joakim Lundeberg; Afshin Ahmadian; Mats Nilsson; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; trinucleotide threading; multiplex amplification; expression profiling; microarray; generic tag; short tandem repeat; microsatellite; electrophoresis; single nucleotide polymorphism; allelotyping; 454; Pyrosequencing; Genetic engineering including functional genomics; Genteknik inkl. funktionsgenomik;

   Sammanfattning : DNA is the code for all life. Via intermediary RNA the information encoded by the genome is relayed to proteins executing the various functions in a cell. Together, this repertoire of inherently linked biological macromolecules determines all characteristics and features of a cell. LÄS MER

7. 15. Studies of immune responses to cell surface proteins of Helicobacter pylori and Borrelia burgdorferi by enzyme immunoassay and immunoblotting

   Författare :Ingrid Nilsson; Avdelningen för medicinsk mikrobiologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mikrobiologi; mycology; virology; B. burgdorferi; H.pylori; cell surface proteins; gel-electrophoresis; enzyme immunoassay; Western immunoblotting; bacteriology; Microbiology; bakteriologi; virologi; mykologi;

   Sammanfattning : Helicobacter pylori and Borrelia burgdorferi s.l. can cause chronic infections by evading the immune system. These two human pathogens express a number of immunogenic cell surface proteins. LÄS MER