Sökning: "DNA library preparation"

Visar resultat 1 - 5 av 13 avhandlingar innehållade orden DNA library preparation.

1. 1. Library Preparation for High Throughput DNA Sequencing

   Författare :Anders Jemt; Joakim Lundeberg; Petri Auvinen; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; RNA; sequencing; massively parallel sequencing; library preparation; automation; genome; transcriptome; Bioteknologi; Biotechnology;

   Sammanfattning : Order 3 billion base pairs of DNA in the correct order and you get the blueprint of a human, the genome. Before the introduction of massively parallel sequencing a little more than a decade ago it would cost around $10 million to get this blueprint. LÄS MER

3. 2. Advances in DNA Detection

   Författare :Yajing Song; Olof Emanuelsson; Jonas Tegenfeldt; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; DNA detection; active filter paper; visual detection; throughput; fluorescence; superparamagnetic beads; rare DNA; Q-PCR; chemical fragmentation; DNA library preparation; massively parallel sequencing; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : DNA detection technologies have an increasing importance in our everyday lives, with applications ranging from microbial diagnostics to forensic analysis, food safety evaluation, and environmental monitoring. Currently, as the associated costs decrease, DNA diagnostic techniques are routinely used in research laboratories, in clinical and forensic practice. LÄS MER

4. 3. Methods to Prepare DNA for Efficient Massive Sequencing

   Författare :Sverker Lundin; Joakim Lundeberg; Harold Swerdlow; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA; Massive sequencing; Next Generation Sequencing; Library Preparation; Barcoding; Multiplexing;

   Sammanfattning : Massive sequencing has transformed the field of genome biology due to the continuous introduction and evolution of new methods. In recent years, the technologies available to read through genomes have undergone an unprecedented rate of development in terms of cost-reduction. LÄS MER

5. 4. Enabling massive genomic and transcriptomic analysis

   Författare :Henrik Stranneheim; Joakim Lundeberg; Elaine Mardis; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; RNA; sequencing; massively parallel sequencing; alignment; assembly; single nucleotide polymorphism; LHON;

   Sammanfattning : In recent years there have been tremendous advances in our ability to rapidly and cost-effectively sequence DNA. This has revolutionized the fields of genetics and biology, leading to a deeper understanding of the molecular events in life processes. LÄS MER

7. 5. Methods for deep examination of DNA

   Författare :Mårten Neiman; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :massive parallel sequencing;

   Sammanfattning : The development of sequencing technology has had a rapid pace during the last years and today, the sequencing instruments harbors enormous capacity. This thesis is about the development of methods to make the most out of this capacity and to use it for various applications. LÄS MER