Sökning: "Biomolecular methods"

Visar resultat 1 - 5 av 66 avhandlingar innehållade orden Biomolecular methods.

  1. 1. Modelling biomolecular interactions

    Författare :Johan Bredenberg; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Computational chemistry; molecular dynamics; nuclear receptors; force field parameterization; zinc fingers; DNA; proteins.;

    Sammanfattning : Computational approaches for understanding and aiding in molecular biology has increased in significance over the last decades, where a wealth of biochemical experiments have provided a solid ground for developing computer models that can be used to predict unresolved issues within biology. Molecular dynamics (MD) is one of these methods, based on classical laws, and suitable for handling large macromolecules in their natural environment, water. LÄS MER

  3. 2. Probing biomolecular fragmentation

    Författare :Nicole Haag; Henrik Cederquist; Henning T. Schmidt; Paul Scheier; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Physics; Fysik; fysik; Physics;

    Sammanfattning : This thesis deals with fragmentation of complex molecular ions, especially biomolecules, in gas phase collision experiments. The aim is to investigate the relations between energy deposition and fragmentation and to shed light on the mechanisms behind energy and charge transfer processes in collisions involving the building blocks of life. LÄS MER

  4. 3. QCM-D – with focus on biosensing in biomolecular and cellular systems

    Författare :Erik Nilebäck; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; QCM-D; biomolecular interactions; cell-surface interaction; glycosaminoglycans; viscoelasticity; cell morphology; Quartz crystal microbalance with dissipation monitoring; surface chemistry; protein conformation;

    Sammanfattning : The high degree of complexity in biological systems is being investigated in greater and greater detail by increasingly sophisticated sensing approaches. The quartz crystal microbalance with dissipation monitoring (QCM-D) technique stands out in this context, by probing the viscoelastic properties at interfaces. LÄS MER

  5. 4. Fluorescence-based methods to probe long-lived transient states for biomolecular studies

    Författare :Heike Hevekerl; Jerker Widengren; Martin Hof; KTH; []
    Nyckelord :Fysik; Physics;

    Sammanfattning : Fluorescence spectroscopy and imaging are wide-spread tools in life science. Themain read-out parameters are still fluorescence intensity and wavelength, but given thebenefits of multi-parameter characterization there are also good reasons to consideradditional fluorescence-based read-out parameters. LÄS MER

  7. 5. Structure determination of ribosomal proteins and development of new methods in biomolecular NMR

    Författare :Magnus Helgstrand; KTH; []
    Nyckelord :nuclear magnetic resonance; structure determination; ribosomal proteins; NMR simulations; NMR theory; NMR assignment software; semiautomatic assignment;

    Sammanfattning : This thesis concerns different areas of biomolecular nuclearmagnetic resonance spectroscopy (NMR). In the first part of thethesis a new formalism for simulations of NMR pulse sequencesis introduced. The formalism is derived both from classicalmechanics and quantum mechanics and is presented forhomonuclear and heteronuclear spin systems. LÄS MER