Sökning: "error hot spots"

Hittade 2 avhandlingar innehållade orden error hot spots.

  1. 1. Accuracy of mRNA Translation in Bacterial Protein Synthesis

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Jingji Zhang; Måns Ehrenberg; Olke C. Uhlenbeck; [2015]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein synthesis; genetic code; misreading; error hot spots; kinetics; aminoglycoside; Biokemi; Biochemistry;

    Sammanfattning : Reading of messenger RNA (mRNA) by aminoacyl-tRNAs (aa-tRNAs) on the ribosomes in the bacterial cell occurs with high accuracy. It follows from the physical chemistry of enzymatic reactions that there must be a trade-off between rate and accuracy of initial tRNA selection in protein synthesis: when the current accuracy, the A-value, approaches its maximal possible value, the d-value, the kinetic efficiency of the reaction approaches zero. LÄS MER

  2. 2. Rate and Accuracy of Bacterial Protein Synthesis with Natural and Unnatural Amino Acids

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Ka-Weng Ieong; Måns Ehrenberg; Simpson Joseph; [2014]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ribosome; protein synthesis; translation; efficiency-accuracy trade-off; kinetics; unnatural amino acids; Molecular Biology; Molekylärbiologi;

    Sammanfattning : This thesis addresses different questions regarding the rate, efficiency, and accuracy of peptide bond formation with natural as well as unnatural amino acids: Which step is rate-limiting during peptide bond formation? How does the accuracy vary with different transfer RNAs (tRNAs) and codons and how is it relevant to the living cells? Does proofreading selection of codon reading occur in a single- or multi-step manner as theoretically suggested? How does the E. coli translation system discriminate unnatural amino acids? Based on that, how to improve the incorporation efficiencies of unnatural amino acids?Based on the study on pH dependence of peptide bond formation, we show that the rate of the chemistry of peptidyl transfer to aminoacyl-tRNA (AA-tRNA) Gly-tRNAGly or Pro-tRNAPro limits the rate of peptide bond formation at physiological pH 7. LÄS MER