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Hittade 4 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Accuracy of mRNA Translation in Bacterial Protein Synthesis

   Författare :Jingji Zhang; Måns Ehrenberg; Olke C. Uhlenbeck; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein synthesis; genetic code; misreading; error hot spots; kinetics; aminoglycoside; Biokemi; Biochemistry;

   Sammanfattning : Reading of messenger RNA (mRNA) by aminoacyl-tRNAs (aa-tRNAs) on the ribosomes in the bacterial cell occurs with high accuracy. It follows from the physical chemistry of enzymatic reactions that there must be a trade-off between rate and accuracy of initial tRNA selection in protein synthesis: when the current accuracy, the A-value, approaches its maximal possible value, the d-value, the kinetic efficiency of the reaction approaches zero. LÄS MER

3. 2. Urban Building Energy Modeling for Retrofit Scenarios : Development, Calibration, Validation and Implementation for Swedish Residential Buildings

   Författare :Fatemeh Johari; Joakim Widén; Darren Robinson; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Urban building energy modeling; Urban building energy retrofit; Urban energy system; Urban energy planning; Energy performance certificate; Energy efficiency; low-energy cities; Engineering Science with specialization in Civil Engineering and Built Environment; Teknisk fysik med inriktning mot byggteknik och byggd miljö;

   Sammanfattning : The necessity for an accelerated transition of urban energy systems and, in particular, the building sector toward energy efficiency and carbon neutrality poses new challenges to planning and retrofitting existing buildings. To cope with these challenges, so-called urban building energy models (UBEMs) have been introduced for quantifying the energy demand in the building sector, identifying the hot spots of energy use and suggesting scenarios for retrofitting the buildings. LÄS MER

4. 3. Rate and Accuracy of Bacterial Protein Synthesis with Natural and Unnatural Amino Acids

   Författare :Ka-Weng Ieong; Måns Ehrenberg; Simpson Joseph; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ribosome; protein synthesis; translation; efficiency-accuracy trade-off; kinetics; unnatural amino acids; Molecular Biology; Molekylärbiologi;

   Sammanfattning : This thesis addresses different questions regarding the rate, efficiency, and accuracy of peptide bond formation with natural as well as unnatural amino acids: Which step is rate-limiting during peptide bond formation? How does the accuracy vary with different transfer RNAs (tRNAs) and codons and how is it relevant to the living cells? Does proofreading selection of codon reading occur in a single- or multi-step manner as theoretically suggested? How does the E. coli translation system discriminate unnatural amino acids? Based on that, how to improve the incorporation efficiencies of unnatural amino acids?Based on the study on pH dependence of peptide bond formation, we show that the rate of the chemistry of peptidyl transfer to aminoacyl-tRNA (AA-tRNA) Gly-tRNAGly or Pro-tRNAPro limits the rate of peptide bond formation at physiological pH 7. LÄS MER

5. 4. Risk factors of road traffic injuries among motorcyclists in Dar es Salaam, Tanzania

   Författare :Filbert Francis; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Background: Commercial motorcycling has become an alternative mode of transport and employment in low and middle-income countries, contributing to road traffic injuries and deaths. Still, there is limited context-specific knowledge regarding risk factors associated with commercial motorcycling. LÄS MER