Sökning: "broadly targeted pcr"
Hittade 5 avhandlingar innehållade orden broadly targeted pcr.
1. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies
Sammanfattning : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. LÄS MER
2. Detection and Quantification of Variable Viral RNA by Real-Time PCR Assays
Sammanfattning : As the area of nucleic acid based technologies develops, so will our understanding of how structural variations in DNA and RNA pathogens are associated with disease. The overall goal of this thesis is the development of broadly targeted measurement techniques for variable viral RNA by Real-Time PCR (here referred to as quantitative reverse transcriptase PCR, QRT-PCR). LÄS MER
3. Molecular Diagnosis of Common Viral Infectious Diseases Based on Real-Time PCR
Sammanfattning : Molecular biology has become an integral part of the diagnosis of infectious diseases. Recently, quantitative real-time PCR (QPCR) methods (often in the form of so-called TaqMan® systems) have been developed for the diagnosis of a wide range of infectious diseases; these techniques found valuable clinical application in the diagnosis and evaluation of progress and therapeutic success of viral diseases. LÄS MER
4. Repertoire diversity and maturation of HIV-1 vaccine-induced B cell responses
Sammanfattning : In the work presented in this thesis, I characterized B cell responses in rhesus macaques inoculated with HIV-1 envelope glycoprotein (Env)-based vaccines to define genetic and functional properties of the elicited antibodies for an improved understanding of Env immunogenicity. In Paper I, we investigated antibody heavy chain V gene segment expression in the overall B cell repertoire and in response to vaccination by using 2 approaches, 454-pyrosequencing and single-cell RT-PCR of bulk and sorted memory B cells, respectively. LÄS MER
5. New molecular markers in mantle cell lymphoma : studies of cannabinoid receptors, 5-lipoxygenase and SOX11
Sammanfattning : Mantle cell lymphoma (MCL) is a malignant B-cell lymphoma that accounts for 5-10% of all lymphomas and tends to occur in older adults with a higher incidence in males. The genetic hallmark of this neoplasm is the t(11;14) translocation which results in overexpression of nuclear cyclin D1. LÄS MER