Sökning: "thermolabile UNG"

Hittade 2 avhandlingar innehållade orden thermolabile UNG.

1. 1. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies

   Författare :Amal Elfaitouri; Jonas Blomberg; Helene Norder; Uppsala universitet; []
   Nyckelord :Microbiology; Real-time PCR; broadly targeted PCR; degenerated PCR; rTth DNA polymerase; thermolabile UNG; QPIA; IgM; Mikrobiologi;

   Sammanfattning : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. LÄS MER

3. 2. Molecular Diagnosis of Common Viral Infectious Diseases Based on Real-Time PCR

   Författare :Nahla Mohamed; Jonas Blomberg; Jan Fohlman; Elgh Fredrik; Uppsala universitet; []
   Nyckelord :Molecular biology; Microbiology; Virology; rTth DNA polymerase; UNG; QPCR; RNA virus; broadly targeted PCR; Molekylärbiologi;

   Sammanfattning : Molecular biology has become an integral part of the diagnosis of infectious diseases. Recently, quantitative real-time PCR (QPCR) methods (often in the form of so-called TaqMan® systems) have been developed for the diagnosis of a wide range of infectious diseases; these techniques found valuable clinical application in the diagnosis and evaluation of progress and therapeutic success of viral diseases. LÄS MER