Sökning: "aspartic protease"

Visar resultat 21 - 25 av 25 avhandlingar innehållade orden aspartic protease.

  1. 21. Molecular Simulation of Enzyme Catalysis and Inhibition

    Författare :Sinisa Bjelic; Johan Åqvist; Adrian J. Mulholland; Uppsala universitet; []
    Nyckelord :Theoretical chemistry; enzyme catalysis; enzyme inhibition; computer simulations; molecular dynamics; empirical valence bond method; structure-based inhibitor design; Teoretisk kemi;

    Sammanfattning : The reaction mechanisms for the hemoglobin degrading enzymes in the Plasmodium falciparum malaria parasite, plasmepsin II (Plm II) and histo-aspartic protease (HAP), have been analyzed by molecular simulations. The reaction free energy profiles, calculated by the empirical valence bond (EVB) method in combination with molecular dynamics (MD) and free energy perturbation (FEP) simulations are in good agreement with experimental data. LÄS MER

  3. 22. Protein Interaction Studies with Low Molecular Weight Ligands : Applications for Drug Discovery, Basic Research and Diagnostic Tool Design

    Författare :Tony Christopeit; U.Helena Danielson; Danièle Altschuh; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : In this thesis, the interactions between different proteins and small ligands were characterized by surface plasmon resonance spectroscopy (SPR) and fluorescence resonance energy transfer (FRET) based assays.   For the C-reactive protein (CRP), a new type of artificial binder was identified which allows designing diagnostic assays superior to commonly used standard assays. LÄS MER

  4. 23. Unravelling the structure and dynamics of lipid nanoparticles for biomolecule delivery

    Författare :Jennifer Gilbert; NanoLund: Centre for Nanoscience; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; lipid nanoparticles; neutron scattering; SAXS; protein encapsulation; nucleic acid delivery; lipid self-assembly;

    Sammanfattning : Encapsulation in self-assembled lipid structures has been shown to increase the stability of large biomolecules, including proteins and nucleic acids (NAs), which has many potential applications in the food and pharmaceutical industries. In order to fully optimise the encapsulation system for a certain application, however, it is important to understand the effect of the encapsulated biomolecule on the lipid structure and vice versa. LÄS MER

  5. 24. Immunocytochemical visualization of cathepsin D during oxidative stress

    Författare :Karin Roberg; Linköpings universitet; []
    Nyckelord :MEDICINE; MEDICIN;

    Sammanfattning : Cathepsin D is the major aspartic protease in lysosomes, and it is found in almost all animal cells. In the present studies, a pre-embedding immunocytochemical method was used to visualize cathepsin D in cultured cells. The protein can be detected at both light and electron microscopic levels by neutral-pH silver enhancement of ultrasmall (0. LÄS MER

  7. 25. Matrix degrading proteases in vascular disease

    Författare :Sofia Jormsjö-Pettersson; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Atherosclerosis; abdominal aortic aneurysm; MMP; cathepsin; polymorphism; in vitro; proteases; extracellular matrix.;

    Sammanfattning : Diverse processes of vascular disease including lesion growth, plaque rupture and abdominal aortic aneurysm formation are influenced by protease expression. Arteries undergo compensatory remodelling when the mechanical forces that are imposed on them are altered. LÄS MER