Sökning: "Strand break"

Visar resultat 16 - 20 av 44 avhandlingar innehållade orden Strand break.

  1. 16. Homologous recombination at replication forks in mammalian cells

    Författare :Cecilia Lundin; Thomas Helleday; Klaus Erixon; Keith W. Caldecott; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Genetics; Genetik;

    Sammanfattning : Accumulating evidence indicates that homologous recombination plays an important role in mammalian cells, primarily in the reactivation of stalled replication forks. During a normal round of replication, stalling of the replication fork occurs frequently when a nick or a lesion, which cannot be by-passed, is encountered. LÄS MER

  3. 17. Mineral fibers, cigarette smoke, and oxidative DNA damage : An experimental study

    Författare :Per Leandersson; Östergötlands Läns Landsting; []
    Nyckelord :MEDICINE; MEDICIN;

    Sammanfattning : This study concerns the ability of mineral fibers and cigarette smoke to generate reactive oxygen metabolites and to cause damage to DNA. The generation of hydroxyl radicals (OH") was demonstrated by using the DNA base, deoxyguanosine (dG) as a trapping agent for OH" and determining its hydroxylation to 8-hydroxydeoxyguanosine (80HdG), and oxidative DNA damage was assessed as formation of 80HdG in isolated DNA or in DNA of human lung cells in culture. LÄS MER

  4. 18. The role of the cohesin loader in genome stability : a journey from yeast to human

    Författare :Fosco Giordano; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Structural Maintenance of Chromosome (SMC) complexes, as their name suggests, have a central role in maintaining the higher structure of genomes, from bacteria to human, and in doing so protecting their integrity. Cohesin, one of three SMC complexes, is required to hold sister chromatids together until anaphase, and for homologous recombination-based DNA repair. LÄS MER

  5. 19. DNA repair and genome organisation in mammalian cells : studies involving single-cell electrophoresis

    Författare :Stefan Eriksson; Bert van Zeeland; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Physical Biology; fysikalisk biologi;

    Sammanfattning : The comet assay (single-cell electrophoresis) is a comparably new method for measurement of DNA strand breakage, primarily in eukaryotic cells. Its main advantage is that it works on the single-cell level, making it possible to detect heterogeneity in DNA damage induction and repair. LÄS MER

  7. 20. Maintenance of genome integrity via activities of the cohesin network

    Författare :Emma Lindgren; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Correct segregation of sister chromatids is an important mechanism for keeping the genome intact. The cohesin complex holds the sister chromatids together from the time of their formation during replication, until separation at anaphase and is thereby mediating cohesion between the sister chromatids, essential for correct chromatid segregation. LÄS MER