Sökning: "Enzyme characterization"

Visar resultat 1 - 5 av 227 avhandlingar innehållade orden Enzyme characterization.

  1. 1. Enzyme assays for lignin–carbohydrate bond hydrolysis

    Författare :Hampus Sunner; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Enzyme kinetic param- eter estimation; Glucuronoyl esterase; Enzyme assays; Lignin-carbohydrate bonds; Synergistic enzyme assay;

    Sammanfattning : To identify, produce, and use enzymes, analytical methods known as enzyme assays are employed. Enzyme assays are based on analysing the changes brought about on a substrate by an enzyme under defined conditions. Assays are often based on simplified reactions acting as substitutes for the reaction of interest. LÄS MER

  3. 2. Oxidation of 1,2-Diols Using Alcohol Dehydrogenases : From Kinetic Characterization to Directed Evolution

    Författare :Cecilia Blikstad; Mikael Widersten; Stefan Lutz; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; enzyme kinetics; alcohol dehydrogenase; directed evolution; enzyme engineering; diol; α-hydroxy aldehyde; Biokemi; Biochemistry;

    Sammanfattning : The use of enzymes as catalysts for chemical transformations has emerged as a “greener” alternative to traditional organic synthesis. An issue to solve though, is that enzymes are designed by nature to catalyze reactions in a living cell and therefore, in many cases, do not meet the requirements of a suitable biocatalyst. LÄS MER

  4. 3. Baeyer-Villiger Monooxygenases from a Dietzia sp. - Enzyme Discovery, Characterization and Engineering

    Författare :Serena Bisagni; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Dietzia; Baeyer-Villiger monooxygenases; biocatalysis; enzyme;

    Sammanfattning : With the emergence of Green Chemistry, biocatalysis is becoming an important approach in many laboratory and industrial processes. Enzymes catalyse chemical reactions with high regio- and stereoselectivity at mild conditions and, most importantly, are able to form products that are not possible to obtain by conventional synthetic chemistry. LÄS MER

  5. 4. Towards Classification and Functional Description of Enzymes: A case study of feruloyl esterases

    Författare :Gupta Udatha; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; descriptors; enzyme immobilization; catalytic triad; pharmacophore; enzyme promiscuity; functional classification; molecular docking; feruloyl esterases; protein evolution; structure-function relationship;

    Sammanfattning : The prediction of enzyme functionality from sequence or structure data remains a challenging task that can be best addressed by studying the structure-function relationships determined from previously available information. This thesis work was focused on developing a reliable classification and functional description for the feruloyl esterase (FAE) enzyme family, whose members’ possess both structural and catalytic promiscuity. LÄS MER

  7. 5. New Methods for Enzyme Hydrolysis, Analysis, and Characterization of Modified Cellulose

    Författare :Claes Melander; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytisk kemi; Analytical chemistry; Methyl Cellulose; Reducing End; Micro Immobilised Enzyme Reactor; Mass Spectrometry; Enzyme Hydrolysis; Modified Cellulose; Substituent Distribution;

    Sammanfattning : Cellulose can be chemically modified to produce derivatives with specific properties. Knowledge of correlations between the modification reaction, substituent distribution, origin of cellulose and properties are importaint in order to design processess so as to produce derivatives with the desired properties. LÄS MER