Sökning: "trypsin"

Visar resultat 16 - 20 av 91 avhandlingar innehållade ordet trypsin.

  1. 16. C-reactive protein in clinical practice with special regard to infectious diseases

    Författare :Lars-Olof Hansson; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :CRP; general practice; septi-caemia; endocarditis; influenza; bacterialmeningitis; viral meningitis; community-acquired pneumonia; malignant melanoma; albumin; transferrin; a-1 anti-trypsin; hapto-globin; orosomucoid; transthyretin; iron.;

    Sammanfattning : A new simple affinity purification method for human CRP using Arginine-Sepharose 4B was developed with a recovery of >90 % and containing < 5-10 % contaminating proteins. In a multicenter study including 607 consecutive patients in general practice, erythrocyte sedimentation rate (ESR) and serum CRP were compared. LÄS MER

  3. 17. Aspects of proteinase-activated receptor-2: A sensor of enzymatic activity at the cell surface

    Författare :Anna-Karin Alm; Institutionen för experimentell medicinsk vetenskap; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; neuropsychology; Neurology; endometrium.; coagulation; signalling; proteinase; G-protein; cell; receptor; neurophysiology; Neurologi; neuropsykologi; neurofysiologi;

    Sammanfattning : Information about extracellular proteolytic activity is transduced to the cell by a transmembrane G-protein coupled receptor subfamily. These receptors are activated by proteolytic cleavage in the extracellular amino-terminal domain and are thus termed proteinase-activated receptors (PARs). LÄS MER

  4. 18. Studies of a Proteinase-activated Cell Surface Receptor

    Författare :Sverker Nystedt; Institutionen för experimentell medicinsk vetenskap; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; receptor; proteinase; PAR-2; molecular cloning; in situ hybridisation; Expression; G-protein; Neurology; neuropsychology; neurophysiology; Neurologi; neuropsykologi; neurofysiologi;

    Sammanfattning : The G protein-coupled receptors form one of the largest families of proteins involved in signalling between cells. It contains receptors for molecules as diverse as biogenic amines, glycoproteins and enzymes. The manuscripts included in this thesis together describe the initial characterisation of a previously unknown receptor of this class. LÄS MER

  5. 19. Miniaturized Techniques for Protein Analysis

    Författare :Johan Sjödahl; KTH; []
    Nyckelord :Proteins; peptides; proteases; Antarctic krill; Euphausia superba; capillary electrophoresis; fluorosurfactants; mass spectrometry; nanoelectrospray; ESI; MALDI; chip; high-throughput; reproducibility; sensitivity; mass accuracy;

    Sammanfattning : Proteins are a highly diversified group of molecules, andfor their study, advanced analytical tools are required. Inparticular, a need for high-throughput techniques has emergedin order to enable the characterization of large sets ofproteins. LÄS MER

  7. 20. Membrane protein proteomics - Novel method for membrane protein identification and quantification

    Författare :Maria Bentz; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biokemisk teknik; mebran; steroider; Lipider; membranes; steroids; Lipids; enzymologi; Proteiner; Membrane proteins; Proteomics; Proteinase K; N-terminal labelling; Relative Quantitation; Database search; Cancer; Cytology; oncology; cancerology; Cytologi; onkologi; cancer; enzymology; Proteins; Biochemical technology;

    Sammanfattning : Membrane proteins are fairly refractory to digestion especially by trypsin. Less specific proteases like elastase and pepsin are much more effective. However database searching using non-tryptic peptides is much less effective due to the lack of charge localisation at the N- and C-termini and the absence of sequence specificity. LÄS MER