Sökning: "fluorescent probe"

Visar resultat 1 - 5 av 66 avhandlingar innehållade orden fluorescent probe.

1. 1. The Light-up Probe

   Författare :Nicke Svanvik; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; asymmetric cyanine dyes; light-up probes; PNA; real-time PCR; LightUp Probe; fluorescent hybridization probes; homogeneous hybridization assays;

   Sammanfattning : The work presented in this thesis concerns the development of light-up probes and their potential applications in PCR based molecular diagnostics. The light-up probe is a nucleic acid analog (here PNA) oligomer linked to an asymmetric cyanine dye (here thiazole orange) and it combines the excellent hybridization properties of PNA with the large fluorescence enhancement of the dye upon binding to DNA. LÄS MER

3. 2. Tailoring Fluorescent Probes for Organelle-Specific Imaging and Sensing

   Författare :Xin Zhang; Zhangjun Hu; Kajsa Uvdal; Fredrik Almquist; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Fluorescent imaging; Ratiometric Nanoprobe; Autophagy; Apoptosis; FRET; Fluorescerande avbildning; Ratiometric Nanoprobe; Autophagy; Apoptosis; FRET;

   Sammanfattning : Exploring and visualizing biological and pathological processes in living cells are useful for both fundamental research and applications. An understanding on the molecular level for these processes with focus on tracking biomarkers, collecting information about their surroundings, and uncovering essential molecular pathways and functions in live cells are of vital interest for cell biological study and clinical diagnosis application. LÄS MER

4. 3. Synthesis and Applications of Dynamic Multivalent Nanostructures

   Författare :Kitjanit Neranon; Olof Ramström; Aldrik Velders; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; multivalent nanostructures; self-assembly; metal-ligand exchange; dynamic covalent chemistry; bipyridine derivatives; dendrimers; liposomes; drug delivery; antimicrobial materials; fluorescent probe; plasmonic chemistry; gold surface functionalization; Kemi; Chemistry;

   Sammanfattning : This thesis focuses on the design, synthesis and development of dynamic multivalent nanostructures such as supramolecular dendrimers, liposomes and gold-functionalized nanostructures. These structures can be used for drug delivery and molecular sensing applications. LÄS MER

5. 4. Salinomycin analogs : Mechanistic probes and structure–activity relationships against cancer stem cells

   Författare :Björn Borgström; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; salinomycin; semi–synthesis; ionophore properties; cancer stem cells; fluorescent probe;

   Sammanfattning : Cancer stem cells are a subpopulation of cells that possess an increased tumorigenic potential. Such cells are connected to drug resistance, metastasis, and cancer recurrence. In 2009, the polyether ionophore salinomycin was shown to exhibit selective activity against cancer stem cells. LÄS MER

7. 5. Development of Fluorescent Nucleobase Analogues - Intrinsically labelled nucleic acids for molecular binding investigations

   Författare :Mattias Bood; Göteborgs universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; RNA; Fluorescent nucleobase analogue; FRET; pre-microRNA; isothermal titration calorimetry; surface plasmon resonance; aminoglycoside; binding interaction;

   Sammanfattning : This thesis focuses on the design, synthesis and utilization of fluorescent nucleobase analogues (FBAs). FBAs are an important class of compounds, used in the research of nucleic acids. The class of canonical FBAs, i.e. LÄS MER