Sökning: "FRET"

Visar resultat 1 - 5 av 58 avhandlingar innehållade ordet FRET.

  1. 1. Förster Resonance Energy Transfer - from single molecule spectroscopy to imaging

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Evangelos Sisamakis; KTH.; [2012]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; FRET; FCS; single-molecule biophysics;

    Sammanfattning : During the last fifteen years several methods have been developed for probing biomolecules (DNA, RNA, proteins) one at a time. Among these methods fluorescence spectroscopy and in particular its many implementations for monitoring Förster Resonance Energy Transfer (FRET), have attracted much interest. LÄS MER

  2. 2. Fluorescence-based ligand assays for protein detection using affibody affinity proteins

    Detta är en avhandling från Stockholm : KTH

    Författare :Björn Renberg; KTH.; [2006]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Affibody molecules; biosensors; FRET; immobilisation; solid-phase synthesis; protein microarrays; NATURAL SCIENCES Chemistry Biochemistry; NATURVETENSKAP Kemi Biokemi;

    Sammanfattning : The detection and quantification of biomolecules, and proteins in particular, are of great interest since these molecules are of fundamental importance to our well-being. Body fluids, as for instance human blood, are well suited for sampling of protein levels. LÄS MER

  3. 3. Adsorption of biopolymers and their layer-by-layer assemblies on hydrophilic surfaces

    Detta är en avhandling från Stockholm : KTH

    Författare :Maria Lundin; KTH.; [2009]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Layer-by-layer; multilayer; adsorption; biopolymers; Chitosan; Heparin; Mucin; Albumin; Lysozyme; β-casein; SDS; QCM-D; Ellipsometri; DPI; TIRF; FRET; SFA; layer structure; solvent content; vertical diffusion; exponential growth; solid-liquid interface; deposition conditions; NATURAL SCIENCES Chemistry Physical chemistry Surface and colloid chemistry; NATURVETENSKAP Kemi Fysikalisk kemi Yt- och kolloidkemi;

    Sammanfattning : It is widely known that surfaces play an important role in numerous biological processes and technological applications. Thus, being able to modify surface properties provides an opportunity to control many phenomena occurring at interfaces. LÄS MER

  4. 4. Fluorescent Nucleobase Analogues and their use for Investigating DNA Interactions

    Detta är en avhandling från Chalmers University of Technology

    Författare :Anke Dierckx; Chalmers tekniska högskola.; Chalmers University of Technology.; [2014]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Fluorescence quantum yield; B-DNA; fluorescent nucleobase analogue; duplex stability; circular dichroism; adenine analogue; FRET;

    Sammanfattning : Ever since unravelling the structure of DNA, an expanding research field has emerged with ongoing efforts dedicated to increase our understanding of the molecule of life. Since the natural nucleobases are virtually non-emissive, it has been a challenge for decades to ‘light up’ DNA/RNA in order to investigate their properties utilizing fluorescence techniques. LÄS MER

  5. 5. DNA-Controlled Lipid-Membrane Fusion

    Detta är en avhandling från Chalmers University of Technology

    Författare :Lisa Simonsson; Chalmers tekniska högskola.; Chalmers University of Technology.; [2009]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biofysik; Biophysics; biomembranes; membrane fusion; vesicles; lipid bilayer; self-assembly; DNA; site-specificity; diffusion; model system; exocytosis; total internal reflection fluorescence microscopy; TIRF microscopy; fluorescence resonance energy transfer; FRET;

    Sammanfattning : Membrane fusion is essential for nerve-cell communication, for protein transport between cell organelles and the cell-membrane and for enabling the merger between virus and host membranes during virus infection. We have demonstrated that short DNA oligonucleotides, membrane-attached via CH in an orientation that mimics the overall zipperlike architecture of fusion-inducing proteins, induce fusion of both suspended vesicles and vesicles site-specifically tethered to SLBs. LÄS MER