Sökning: "precision-cut tissue slices"

Hittade 4 avhandlingar innehållade orden precision-cut tissue slices.

  1. 1. Bioengineering and Cell-derived Strategies for Salivary Gland Regeneration

    Författare :Jiao DONG; Lungbioengineering och regeneration; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; extracellular vesicles; precision-cut tissue slices; histology scoring; sustainable histology; salivary gland; human dental pulp stem cells; extracellulära vesiklar; stamceller från mänsklig tandmassa; precisionsskurna vävnadsskivor; Spottkörtel; hållbar histologi och histologipoäng;

    Sammanfattning : Xerostomia (dry mouth symptoms) is a group of incurable debilitating conditions of salivary glands caused by aging, radiation/chemical exposure, or aberrant inflammation in the salivary glands. During this PhD thesis, we aimed to evaluate whether cell-derived strategies (e.g. LÄS MER

  3. 2. Nitric oxide and eicosanoids : significance and interactions during antigen-induced responses in peripheral lung tissue

    Författare :Anna-Karin Larsson; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Nitric oxide; leukotrienes; prostaglandins; histamine; ovalbumin; contractions; distal lung; rat; human; guinea pig; precision-cut lung slices; mast cell;

    Sammanfattning : Asthma means difficulty in breathing and is described as a chronic, inflammatory disorder that produces narrowing of the lower respiratory tract. The allergen-induced asthmatic bronchoconstriction is primarily caused by an IgE-mediated release of the mast cell mediators, histamine and eicosanoids (leukotrienes and prostanoids). LÄS MER

  4. 3. Adrenal Bioactivation and Toxicity of 3-MeSO2-DDE, o,p´-DDD and DMBA Investigated in Tissue Slice Culture

    Författare :Örjan Lindhe; Jan Alexander; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Developmental biology; adrenal cortex; 3-MeSO2-DDE; o; p´-DDD; DMBA; bioactivation; binding; toxic; Utvecklingsbiologi; Developmental biology; Utvecklingsbiologi; Ecotoxicology; ekotoxikologi;

    Sammanfattning : I developed a precision-cut adrenal slice culture procedure to investigate cytochrome P450 (CYP) catalysed irreversible binding and adrenocorticolytic effects in human, rodent, and fish adrenal tissue, ex vivo. Autoradiography and radioluminography of exposed tissue slices showed that the potent adrenal toxicant 3-methylsulphonyl-2,2´-bis(4-chlorophenyl)-1,1´-dichloroethene (MeSO2-DDE) causes a selective metabolite binding in zona fasciculata (ZF), which is diminished by the CYP11B1 inhibitor metyrapone. LÄS MER

  5. 4. Pancreatic cancer : investigation of the prognostic significance of tumor immunophenotype and establishment of a novel ex-vivo tissue slice culture system for drug sensitivity testing

    Författare :Carlos Fernández Moro; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Pancreatic ductal adenocarcinoma (PDAC), commonly referred to as pancreatic cancer, is associated with a dismal 5-year overall survival of 9 to 6 %. A major reason for the high mortality is the pronounced resistance to treatment. LÄS MER