Sökning: "duplex stability"

Visar resultat 21 - 25 av 33 avhandlingar innehållade orden duplex stability.

1. 21. Aspects of Antisense and Antigene Chemistry of Oligonucleotides Tethered to Intercalators

   Författare :Dimitri Ossipov; Jesper Wengel; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Bioorganic chemistry; oligonucleotides; intercalators; ruthenium complexes; RNase H cleavage; photocross-linkage; Bioorganisk kemi; Bioorganic chemistry; Bioorganisk kemi; bioorganisk kemi; Biorganic Chemistry;

   Sammanfattning : Synthetic and physicochemical studies on appropriately functionalized ODN-conjugates have been performed to evaluate their abilities to act as antisense agents against RNA or as intramolecular DNA cross-linking agents. Intercalating aromatic systems [phenazine (Pnz), dipyridophenazine (DPPZ)] and metallointercalators such as Ru2+(phen)2(DPPZ) and Ru2+(tpy)(DPPZ)L [where L = chemically or photochemically labile ligand, phen = phenanthroline, tpy = terpyridine], which are covalently tethered to the oligo-deoxynucleotides (ODNs), have been chosen for this purpose. LÄS MER

3. 22. Studies on nucleic acids chemistry

   Författare :Nitin Puri; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Bioorganic chemistry; Bioorganisk kemi; Bioorganic chemistry; Bioorganisk kemi; bioorganisk kemi; Biorganic Chemistry;

   Sammanfattning : This thesis is divided into three parts: Part 1: Synthesis and conformational analysis of cyclic RNAs, and comparison with lariat RNAs modelling the lariat formed during processing of Group II and Nuclear pre-mRNA. Synthesis of a hexameric lariat RNA model with a tetranucleotidyl "2' → 5' loop" and a dinucleotidyl "3'-tail" has been accomplished. LÄS MER

4. 23. Biosensing platforms using graphene based bioreactive nanostructures with various dimensions

   Författare :Yuanyuan Han; Klaus Leifer; Jöns Hilborn; Hu Li; Boukherroub Rabah; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Graphene; nanoparticles; nanostructure; biosensors;

   Sammanfattning : Nanomaterials have brought new aspects and improvements to the biosensing field due to their unique physical and chemical properties that are not shown in the bulk state. This thesis focuses on concepting, developing and testing of biosensors where nanomaterials including graphene gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs) constitute the biosensors. LÄS MER

5. 24. Applications of Chromophores and Multiphoton Techniques to Study Structure and Interactions of Bio-macromolecules in Assembled State

   Författare :Piotr Hanczyc; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Z-scan; One-photon spectroscopy; oxazole yellow dyes; two-photon absorption.; non-linear spectroscopy; PVA DNA dyes films; linear dichroism; ruthenium complexes;

   Sammanfattning : The research presented in this thesis is concerned with the linear and nonlinear optical properties of biopolymers and the chromophores that bind to them. This thesis combines analyses of the interactions of biomolecules with technological improvements of already existing systems for bionanotechnology-related research. LÄS MER

7. 25. Studies on natural antisense RNAs and microRNAs

   Författare :Omid Reza Faridani; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Regulatory RNAs are found in all kingdoms of life and involved in regulation of gene expression at various steps including RNA splicing, editing, stability, modification, export, translation and chromatin remodelling. A large number of regulatory RNAs have been described recently. LÄS MER