Sökning: "cryo-electron microscopy matrix preparation protein purification zeolites Inorganic chemistry Oorganisk kemi"

Hittade 1 avhandling innehållade orden cryo-electron microscopy matrix preparation protein purification zeolites Inorganic chemistry Oorganisk kemi.

1. 1. Adsorption of proteins on zeolite FAU

   Författare :Daniel Klint; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; matrix preparation; cryo-electron microscopy; protein purification; zeolites; Inorganic chemistry; Oorganisk kemi;

   Sammanfattning : A highly dealuminated form of zeolite FAU, ultra-stable zeolite Y (USY), was used as an adsorbent matrix in the purification of proteins. Undesired proteins were removed from a crude preparation by adsorption on USY, increasing the purity of an unadsorbed protein (horse radish peroxidase), or the protein of interest was adsorbed on the zeolite and subsequently eluted through changes in the solution conditions (hen egg lysozyme, mouse-immunoglobulin G). LÄS MER