Differential expression pattern of CEACAM1 isoforms in polarized epithelial cells, it´s regulation and some functional consequences

Detta är en avhandling från Stockholm : Karolinska Institutet, Department of Cell and Molecular Biology

Sammanfattning: The cell adhesion molecule CEACAM1 is expressed in epithelia, small vessel endothelia and leukocytes as two major, co-expressed isoforms, CEACAM1-L and CEACAM1-S, which differ in their cytoplasmic domains. CEACAM1 has been found both in cell-cell contact areas and on apical surfaces of polarized epithelial cells. The aim of this project was to find out whether CEACAM1-L and CEACAM1S exhibit different surface localization in polarized epithelial cells, and if so to map the signals for the differential surface localization. MDCK cells were used as a model system and were transfected with wildtype and mutant forms of both CEACAM1 -L and CEACAM1-S. We demonstrated that CEACAM1-L was expressed both on the apical and the lateral surfaces of polarized MDCK cells, whereas CEACAM1-S only appeared on the apical surface. We also showed that CEACAM1-L acts as a cell adhesion molecule in the lateral surfaces, binding adjacent cells together by a trans- homophilic complex formation. By double staining with both monoclonal and polyclonal antibodies we could demonstrate that CEACAM1-L occurred in two different conformational states, corresponding to the transhomophilic binding state and a nonbinding state. In CEACAM1-S the trans-homophilic binding state dominated. We found that tyr515 has a crucial role for the lateral localization of CEACAM1-L and that the lysine residues 519-521 as well as ser503 also are important for the steady-state lateral localization. We also demonstrated that both pervanadate, which increases tyrosine phosphorylation, and staurosporine, which decreases serine phosphorylation, caused disappearance of CEACAM1-L from the lateral cell surfaces. By double staining for adaptor proteins AP-1 and AP-2 as well as for markers for early and late endosomes and lysosomes we obtained results suggesting that staurosporine stimulates internalization of CEACAM1-L from the plasma membrane to early and late endosomes and lysosomes, and that pervanadate causes an increased routing of CEACAM1-L from the trans-golgi network to late endosomes and lysosomes. We found no co-localization with the tight junction proteins occludin or ZO-1, but a prominent co-localization of CEACAM1-L with E-cadherin and beta-catenin in the adherens junctions. CEACAM1 -L did not co-localize with desmosomes. However, expression of CEACAM1-L caused a prominent reduction of the amount of desmosomal plaques in the lateral surfaces and a concomitant disorganization of the submembraneous cytokeratin filaments. The signal for desmosomal perturbation was mapped to the C-terminal part of the cytoplasmic domain of CEACAM1. Last we studied the appearance of the two CEACAM1 isoforms in intracellular vesicles and primary cilia. CEACAM1-L but not CEACAM1-S, was found in prominent large tubulovacuolar structures in the subapical part of polarized MDCK cells. CEACAM1 -S was present in small vesicular structures. When the cells became superconfluent they develop primary cilia, that were identified by staining for alpha-tubulin and gamma-tubulin. Both CEACAM1-L and CEACAM1-S, were expressed on the cilia. Especially CEACAM1-S, was localized to the distal ends of the cilia. The distal ends of primary cilia from different cells were often seen in contact with each other, suggesting that CEACAM1 -S mediated adhesion between primary cilia.

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