Sökning: "two-phase partitioning"

Visar resultat 6 - 10 av 33 avhandlingar innehållade orden two-phase partitioning.

1. 6. Lactic acid production: extractive fermentation in aqueous two-phase systems

   Författare :Jordi Planes; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; gel excludion chromatography; polyethylenimine; ethylene oxide propylene oxide; phase forming polymers; aqueous two-phase systems; extractive fermentation; Lactococcus; Lactobacillus; Mikrobiologi; bakteriologi; virologi; mykologi; bacteriology; virology; mycology; Microbiology;

   Sammanfattning : There is an increased interest in the microbial production of lactic acid as a raw material for the synthesis of lactic acid polymers as biodegradable plastics. However, two factors limit the economical competitiveness of lactic acid production by microorganisms: lactic acid inhibition, which decreases both fermentation rate and the maximum lactic acid concentration, and the efficiency in the recovery of lactic acid. LÄS MER

3. 7. Separation Methods in Membrane Proteomics -Integration of Aqueous Two-Phase Partitioning for Enrichment and Pre-Fractionation

   Författare :Henrik Everberg; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Membrane proteins; Proteins; metabolism; Biokemi; Metabolism; Biochemistry; Aqueous two-phase partitioning; enzymology; Proteiner; enzymologi; Proteomics;

   Sammanfattning : The study of the protein complement of a genome is called proteomics. Proteomics can be defined as identification, quantification and characterization of a total set of proteins expressed at different time-points or under different cellular states to provide global information about biological processes. LÄS MER

4. 8. Integration of Aqueous Two-Phase Systems into Recovery Processes for Biomolecules

   Författare :Cecilia Kepka; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteins; proteins; plasmid DNA; large-scale; protein purification; integration; Expression; aqueous two-phase systems; enzymology; Proteiner; enzymologi;

   Sammanfattning : The main scope of this thesis has been to study and develop new purification methods and also to further develop existing methods for purification to enhance their efficacy. Important parameters in the development of the methods are that they should be suitable for scale-up and possible to be integrated into recovery processes. LÄS MER

5. 9. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

   Författare :Cynthia Hassinen; KTH; []
   Nyckelord :E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

   Sammanfattning : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. LÄS MER

7. 10. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

   Författare :Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

   Sammanfattning : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. LÄS MER