Sökning: "two-phase partitioning"

Visar resultat 11 - 15 av 33 avhandlingar innehållade orden two-phase partitioning.

  1. 11. Selective extraction systems for detection and purification of membrane proteins

    Författare :Ulf Sivars; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; partitioning; Aqueous two-phase systems; separation; membrane proteins; detergent; micelle; polymer; Biochemistry; affinity; Metabolism; Biokemi;

    Sammanfattning : Increased understanding of membrane proteins is important for characterization of biochemical processes. Improved membrane protein isolation methods is a key issue for effective functional and structural determination of the large amount of unknown membrane proteins. LÄS MER

  3. 12. Fusion tags for protein partitioning enhancement and process-scale extraction inaqueous two-phase systems

    Författare :Eric Collet; KTH; []
    Nyckelord :;

    Sammanfattning : .... LÄS MER

  4. 13. Surface Properties of Antibodies and their Complexes with Antigens Studied by LLPC

    Författare :Christer Wingren; Institutionen för immunteknologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; ligand-binding.; surface properties; isomerism; conformational changes; antibody; specificity; two-phase partitioning; antigen-binding sites; Biochemistry; Metabolism; Biokemi; metabolism;

    Sammanfattning : The main objective of this thesis was to gain further insight into the relationship between the exposed surfaces and the functional properties of antibodies and their complexes with antigens in solution. To study this relationship, a new technique, liquid-liquid partition chromatography (LLPC) in aqueous two-phase systems, was developed further with respect to sensitivity, selectivity and reproducibility. LÄS MER

  5. 14. Asexual embryogenesis in plants

    Författare :Boel Mårtensson; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Systematic botany; two-phase partitioning; somatic embryogenesis; pollen embryogenesis; plasma membrane; peroxidase; Daucus carota isoperoxidase; Nicotiana tabacum; Ca2 transport; cell shape; calmodulin; taxonomy; morphology; phytogeography; chemotaxonomy. Physiology of nonvascular plants; Systematisk botanik; taxonomi; morfologi; kemotaxonomi. Växters fysiologi inte kärlväxter .;

    Sammanfattning : The capacity of somatic cells and pollen grains or microspores to undergo embryogenesis is an important property used in improvement and regeneration of plants. Furthermore, somatic embryogenesis as well as pollen embryogenesis show many similarities with zygotic embryogenesis and are, thus, good model systems. LÄS MER

  7. 15. Influence of Escherichia coli feedstock properties on the performance of primary protein purification

    Författare :Mattias Råvik; Andres Veide; Åsa Emmer; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Downstream processing; cell surface properties; surface plasmon resonance; contact angle measurements; microelectrophoresis; aqueous two-phase partitioning; filtration; EBA adsorbents; high-pressure homogenisation; Biochemistry; Biokemi;

    Sammanfattning : Abstract The aim of the present study was to increase the understanding of how the cell surface properties affect the performance of unit operations used in primary protein purification. In particular, the purpose was to develop, set up and apply methods for studies of cell surface properties and cell interactions. LÄS MER