Sökning: "Aqueous two-phase systems"

Visar resultat 1 - 5 av 27 avhandlingar innehållade orden Aqueous two-phase systems.

  1. 1. Peptide-tagged proteins in aqueous two-phase systems

    Författare :Anna Nilsson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; Biokemi; metabolism; Biochemistry; green fluorescent protein; cutinase; tryptophan; Aqueous two-phase system; peptide-tagged protein;

    Sammanfattning : This thesis deals with proteins containing peptide tags for improved partitioning in aqueous two-phase systems. Qualitatively the peptide-tagged protein partitioning could be predicted from peptide data, i.e. partitioning trends found for peptides were also found for the peptide-tagged proteins. LÄS MER

  3. 2. Protein Surfaces Probed by Aqueous Two-phase Partitioning

    Författare :Kristina Berggren; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; partitioning; Aqueous two-phase systems; protein surface; peptide; Biochemistry; amino acid residue; Biokemi;

    Sammanfattning : This thesis is concerned primarily with peptide and protein partitioning in aqueous two-phase systems and the connection between partitioning behavior and protein structure. The surface properties of a protein is often crucial for recognition and interaction with other molecules. LÄS MER

  4. 3. Lactic acid production: extractive fermentation in aqueous two-phase systems

    Författare :Jordi Planes; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; gel excludion chromatography; polyethylenimine; ethylene oxide propylene oxide; phase forming polymers; aqueous two-phase systems; extractive fermentation; Lactococcus; Lactobacillus; Mikrobiologi; bakteriologi; virologi; mykologi; bacteriology; virology; mycology; Microbiology;

    Sammanfattning : There is an increased interest in the microbial production of lactic acid as a raw material for the synthesis of lactic acid polymers as biodegradable plastics. However, two factors limit the economical competitiveness of lactic acid production by microorganisms: lactic acid inhibition, which decreases both fermentation rate and the maximum lactic acid concentration, and the efficiency in the recovery of lactic acid. LÄS MER

  5. 4. Integration of Aqueous Two-Phase Systems into Recovery Processes for Biomolecules

    Författare :Cecilia Kepka; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteins; proteins; plasmid DNA; large-scale; protein purification; integration; Expression; aqueous two-phase systems; enzymology; Proteiner; enzymologi;

    Sammanfattning : The main scope of this thesis has been to study and develop new purification methods and also to further develop existing methods for purification to enhance their efficacy. Important parameters in the development of the methods are that they should be suitable for scale-up and possible to be integrated into recovery processes. LÄS MER

  7. 5. Partitioning of biomolecules in thermoseparating polymer-water two-phase systems

    Författare :Hans-Olof Johansson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aqueous two-phase system; temperature-induced phase separation; amino acid and peptide separation; polymer; partitioning; Biology; Biologi;

    Sammanfattning : The partitioning behaviour of different types of molecules in thermoseparating two-phase systems has been studied. A thermoseparating two phase system was formed by heating a water solution, which contained a thermoseparating polymer, above the cloud point temperature. LÄS MER