Sökning: "conformational changes"

Visar resultat 1 - 5 av 155 avhandlingar innehållade orden conformational changes.

  1. 1. Folding and interaction studies of subunits in protein complexes

    Detta är en avhandling från Umeå : Umeå University

    Författare :Ximena Aguilar; Umeå universitet.; [2014]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Macromolecular crowding; cpn10; Mediator; Med25; Dreb2a; VP16; conformational changes; NMR; ITC.; Biochemistry; biokemi; molekylärbiologi; Molecular Biology;

    Sammanfattning : Proteins function as worker molecules in the cell and their natural environment is crowded. How they fold in a cell-like environment and how they recognize their interacting partners in such conditions, are questions that underlie the work of this thesis. LÄS MER

  2. 2. Surface Properties of Antibodies and their Complexes with Antigens Studied by LLPC

    Detta är en avhandling från Department of Biochemistry, Lund University

    Författare :Christer Wingren; Lunds universitet.; Lund University.; [1997]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; ligand-binding.; surface properties; isomerism; conformational changes; antibody; specificity; two-phase partitioning; antigen-binding sites; Biochemistry; Metabolism; Biokemi; metabolism;

    Sammanfattning : The main objective of this thesis was to gain further insight into the relationship between the exposed surfaces and the functional properties of antibodies and their complexes with antigens in solution. To study this relationship, a new technique, liquid-liquid partition chromatography (LLPC) in aqueous two-phase systems, was developed further with respect to sensitivity, selectivity and reproducibility. LÄS MER

  3. 3. A structural study of ribokinase from Escherichia coli

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Jill A. Sigrell; Uppsala universitet.; [1998]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; ATP-binding; conformational changes; kinase; ribose; X-ray crystallography; Molekylärbiologi; NATURAL SCIENCES Biology Cell and molecular biology Molecular biology; NATURVETENSKAP Biologi Cell- och molekylärbiologi Molekylärbiologi; molekylärbiologi; Molecular Biology;

    Sammanfattning : Ribokinase phosphorylates the abundant sugar ribose, using ATP in the presence of magnesium. The phosphorylation is the first reaction in ribose metabolism and the product, ribose-5-phosphate, can enter the pentose phosphate pathway and glycolysis. It can also be used in the synthesis of histidine and purines. LÄS MER

  4. 4. Development of a Novel QCM Technique for Protein Adsorption Studies

    Detta är en avhandling från Chalmers University of Technology

    Författare :Fredrik Höök; Chalmers tekniska högskola.; Chalmers University of Technology.; [1997]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Fysik; Physics; QCM; protein adsorption; dissipation factor; hemoglobin; ferritin; myoglobin; conformational changes; viscoelastic effects;

    Sammanfattning : The first part of this thesis (Papers I and II) is concentrated on the development of a novel quartz crystal microbalance (QCM) technique to study protein adsorption. The second part (Papers III-V) is focused on the application of the technique on protein adsorption using well characterized surface-solution-protein systems. LÄS MER

  5. 5. Improving selectivity in protein extraction by subtle interactions and environmental changes

    Detta är en avhandling från Department of Biotechnology, Lund University

    Författare :Sheryl Fernandes; Lunds universitet.; Lund University.; [2001]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Difference Spectroscopy; Circular dichroism; Biotechnology; Bioteknik; Chemical technology and engineering; Kemiteknik och kemisk teknologi; Fluorescence spectroscopy; Metal ions; Cibacron blue; Polyvinylpyrrolidone; Polyethylene glycol; Triton X-114; His 6 tag; Cold-active ß-lactamase; Cold-active ß-galactosidase; Lactate dehydrogenase; Cutinase; Affinity Extraction; Conformational change; Partial Denaturation; Molecular interaction; Aqueous two-phase systems; Detergent foam;

    Sammanfattning : The challenges for efficient bioprocesses lie in developing and adapting further current technologies or in creating innovative separation technologies. The thesis is based on the studies on protein recovery from crude feedstocks by extraction in aqueous two-phase systems and detergent foam, respectively. LÄS MER