Sökning: "transduction efficiency"

Visar resultat 1 - 5 av 24 avhandlingar innehållade orden transduction efficiency.

  1. 1. Development of Gene Therapy for Hematopoietic Stem Cells using Lentiviral Vectors

    Författare :Niels-Bjarne Woods; Andreas Ooka; Stefan Karlsson; Avdelningen för molekylärmedicin och genterapi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; embryonic stem cell; Hematopoietic stem cell; lentiviral vector; transduction efficiency; transgene expression; multiple vector copy integration; insertional mutagenesis; NOD SCID mouse; cytogenetics; Genetics; extracellulära vätskor; extracellular fluids; cytogenetik; Genetik; Haematology; Hematologi; Lentivirus : genetics; Human; Genetic Vectors; Hematopoietic Stem Cells : metabolism; Animal; Gene Therapy; Leukemia : therapy; Leukemia : metabolism;

    Sammanfattning : Hematopoietic stem cells are an ideal target for genetic manipulation for the purpose of curing hematological disorders as they have the ability to develop into all blood lineages and to self-renew. In this study we demonstrate that lentiviral vectors, based on HIV-1, can efficiently transfer genes into human hematopoietic progenitor and stem cells as assessed following stem cell transplantation in immune compromised mice. LÄS MER

  3. 2. Negative Pressure Wound Therapy - Effects on Sternotomy Wounds and the Intrathoracic Organs

    Författare :Christian Torbrand; Lund Oftalmologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NPWT; negative pressure; vacuum-assisted closure; sternotomy wounds; deep sternal wound infection; mediastinitis; thoracic surgery; pressure transduction; neurohormones; neuropeptides; total heart volume variation; cardiac output; wound contraction; macrodeformation;

    Sammanfattning : Negative pressure wound therapy (NPWT) has been widely adopted for the treatment of deep sternal wound infections due to its excellent clinical outcome. However, the mechanisms of action and effects on hemodynamics have not been thoroughly elucidated. LÄS MER

  4. 3. Membrane Interactions of Arginine-Rich Peptides for the Intracellular Delivery of Gene-Targeted Drugs

    Författare :Per Thoren; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cellular uptake; translocation; protein transduction; circular dichroism; drug delivery; liposomes; resonance energy transfer; PNA; antigene; antisense;

    Sammanfattning : A major obstacle for the therapeutic use of e.g. oligonucleotides is their inherently poor cellular uptake. A recently discovered class of peptides, denoted cell-penetrating peptides, has been shown to traverse cell membranes, even when conjugated to oligonucleotides or even full-length proteins. LÄS MER

  5. 4. Enhancing the efficiency of viral and non-viral gene delivery vectors

    Författare :Safa Bazaz; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Gene therapy, which involves the introduction, alteration, or deletion of genetic material within cells to treat or prevent disease, holds great promise for addressing various genetic disorders and other medical conditions. However, several challenges remain in the field, particularly concerning nucleic acid delivery into target cells. LÄS MER

  7. 5. Novel Culture Strategies and Signal Transduction Pathways of Pluripotent Stem Cells

    Författare :Sara Pijuan Galitó; Cecilia Annerén; Lena Kjellén; Christopher Ward; Uppsala universitet; []
    Nyckelord :Pluripotent stem cells; Inter-alpha inhibitor; culture method; Biologi med inriktning mot molekylär cellbiologi; Biology with specialization in Molecular Cell Biology; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Sammanfattning : Pluripotent stem cells (PSCs) can self-renew indefinitely in culture while maintaining their capacity to differentiate into any cell type of an organism, thus offering novel sources for drug screening, in vitro disease modelling, and cell replacement therapies. However, due to their sensitive nature, many PSC lines are still cultured using undefined components such as serum or serum-derived components, on either feeder cells or complex protein mixes such as Matrigel or gelatine. LÄS MER