Sökning: "immobilized template"

Visar resultat 1 - 5 av 10 avhandlingar innehållade orden immobilized template.

1. 1. Composite Materials in Molecular Imprinting Technology

   Författare :Ecevit Yilmaz; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; immobilized template; molecularly imprinted polymer; composite material; polymerization method; heterogeneous imprinting; surface; morphology; Polymer technology; Polymerteknik; biopolymers;

   Sammanfattning : Molecularly imprinted polymers (MIPs) are artificial plastic materials that mimic antibodies or enzymes. Like natural antibodies or enzymes, molecularly imprinted polymers can selectively bind target molecules or can promote catalytic reactions. LÄS MER

3. 2. Nano- and Micro-sized Molecularly Imprinted Polymer Particles on Solid Surfaces

   Författare :Tripta Kamra; Synkrotronljusfysik; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : Molecularly imprinted polymers (MIPs) are artificial receptors made by imprinting template molecules in a polymer matrix followed by their removal through washing to obtain a specific and selective template cavities. This property of the MIPs have made them a very efficient material for diverse applications such as chromatography, purification, drug sensing, etc. LÄS MER

4. 3. HIV-1 reverse transcriptase activity assays based on monoclonal antibodies to native enzyme : Applications on studies of antiviral substances and immunological variation of reverse transcriptase

   Författare :Ann-Sofie Rytting; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; HIV-1; reverse transcriptase; monoclonal antibody; activity assay; subtype; RT inhibitor; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

   Sammanfattning : Specific detection of HIV-1 reverse transcriptase (RT) activity in crude materials was enhanced using a capture assay for immunopurification and subsequent activity assay. The purification step was achieved by anti-HIV-1 RT monoclonal antibodies (Mabs) immobilized onto a solid carrier. LÄS MER

5. 4. Reverse transcriptase assay for analysis of resistance to anti-HIV drugs and their mechanism of action

   Författare :Xingwu Shao; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :HIV-1; RT; antiviral drugs; NRTls; NNRTls; drug resistance; viral load;

   Sammanfattning : Reverse transcriptase (RT) is a viral enzyme and one of the main targets for drugs against human immunodeficiency virus (HIV). This work presents a set of sensitive non-radioactive RT assays were developed: standard RT inhibition assay (IC assay); RT binding inhibition assay; RTprotein detection assay and template-primer destruction assay or chain termination assay (CT assay). LÄS MER

7. 5. Microfluidic bead-based methods for DNA analysis

   Författare :Aman Russom; Ulf Gyllensten; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; single nucleotide polymorphism; DNA analysis; SNP; microfluidics; pyrosequencing; beads; lab on a chip; hybridization; DASH; microsystem; micro totat analysis system; allele-specific extension; DASH; microcontact printing; Genetik; Clinical genetics; Klinisk genetik;

   Sammanfattning : With the completion of the human genome sequencing project, attention is currently shifting toward understanding how genetic variation, such as single nucleotide polymorphism (SNP), leads to disease. To identify, understand, and control biological mechanisms of living organisms, the enormous amounts of accumulated sequence information must be coupled to faster, cheaper, and more powerful technologies for DNA, RNA, and protein analysis. LÄS MER