Sökning: "Macromolecular crowding"

Visar resultat 1 - 5 av 13 avhandlingar innehållade orden Macromolecular crowding.

  1. 1. Effects of Macromolecular Crowding on Protein Folding : - in-vitro equilibrium and kinetic studies on selected model systems

    Författare :Alexander Christiansen; Pernilla Wittung-Stafshede; Thomas Kiefhaber; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein Folding; macromolecular crowding; excluded volume; spectroscopy; stopped-flow; biokemi; Biochemistry;

    Sammanfattning : Protein folding is the process during which an extended and unstructured polypeptide converts to its compact folded structure that is most often the functional state. The process has been characterized extensively in dilute buffer in-vitro during the last decades but the actual biological place for this process is the inside of living cells. LÄS MER

  3. 2. Macromolecular interactions, Consequences on carbohydrate metabolism

    Författare :Sandra Matic; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; macromolecular interactions; sucrose synthase; macromolecular crowding; cytoskeleton; BY-2 tobacco cells; alamethicin; aqueous polymer two-phase partitioning;

    Sammanfattning : Most cells are crowded with solutes, enzymes, nucleic acids, structural proteins and membranes. Crowding promotes macromolecular interactions and determines major properties of the cellular environment such as viscosity, diffusion and inhomogeneity. LÄS MER

  4. 3. Effect of Macromolecular Crowding on Diffusive Processes

    Författare :Zahedeh Bashardanesh; David van der Spoel; Per Lötstedt; Lars Schäfer; Uppsala universitet; []
    Nyckelord :;

    Sammanfattning : Macromolecular crowding are innate to cellular environment. Understanding their effect on cellular components and processes is essential. This is often neglected in dilute experimental setup both in vitro and in silico. LÄS MER

  5. 4. Peptide folding and local unfolding of SOD1 in the presence of interacting macromolecular crowders - a Monte Carlo approach

    Författare :Anna Bille; Beräkningsbiologi och biologisk fysik - Genomgår omorganisation; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Monte Carlo simulations; protein folding; SOD1; crowding; Fysicumarkivet A:2016:Bille;

    Sammanfattning : A protein chain often folds into a functional, specific three dimensional structure. Failurein this process can in some cases lead to disease, e.g. the misfolding disease ALS. LÄS MER

  7. 5. Protein stability and mobility in live cells : Revelation of the intracellular diffusive interaction organization mechanisms

    Författare :Xin Mu; Mikael Oliveberg; Johan Elf; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; diffusive interactions; macromolecular crowding; protein thermodynamic stability; protein mobility; in-cell NMR; Hofmeister series; Biochemistry; biokemi;

    Sammanfattning : Biochemical processes inside living cells take place in a confined and highly crowded environment. As such, macromolecular crowding, one of the most important physicochemical properties of cytoplasm, is an essential element of cell physiology. LÄS MER