Sökning: "affinity tags"
Visar resultat 16 - 20 av 22 avhandlingar innehållade orden affinity tags.
16. Strategies for Miniaturized Biomarker Detection
Sammanfattning : The aim of this thesis is development and application of different miniaturized strategies for detection of biomarkers. The biomarker PSA (prostate specific antigen), which is prostate cancer specific, has been the main focus of the thesis. LÄS MER
17. Structural and physicochemical studies on metal and porphyrin binding by the Bacillus subtilis ferrochelatase
Sammanfattning : The enzyme ferrochelatase (EC 4.99.1.1) is the terminal enzyme in the biosynthetic pathway of heme b and catalyzes the insertion of Fe(II) into protoporphyrin IX. LÄS MER
18. Expression and purification of membrane proteins: Focus on the G-protein coupled receptor MC4r
Sammanfattning : Membrane proteins are crucial components of the cell and are involved in many biological processes. Recombinant overexpression systems together with different purification methods are necessary to obtain large amounts of purified receptor for biophysical and functional studies. LÄS MER
19. Selective protein functionalisation via enzymatic phosphocholination
Sammanfattning : Proteins are the most abundant biomolecules within a cell and are involved in all biochemical cellular processes ultimately determining cellular function. Therefore, to develop a complete understanding of cellular processes, obtaining knowledge about protein function and interaction at a molecular level is critical. LÄS MER
20. Computational analyses of biological sequences -applications to antibody-based proteomics and gene family characterization
Sammanfattning : Following the completion of the human genome sequence, post-genomic efforts have shifted the focus towards the analysis of the encoded proteome. Several different systematic proteomics approaches have emerged, for instance, antibody-based proteomics initiatives, where antibodies are used to functionally explore the human proteome. LÄS MER