Sökning: "ISET."

Hittade 4 avhandlingar innehållade ordet ISET..

1. 1. Strategies for Miniaturized Biomarker Detection

   Författare :Belinda Adler; Avdelningen för Biomedicinsk teknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; biomarker; miniturization; lab-on-a-chip; proteomics; PSA; microarray; mass spectrometry; MALDI MS; SPE; sample preparation; immunoaffinity; antibody; aptamer; mass tag; ISET.;

   Sammanfattning : The aim of this thesis is development and application of different miniaturized strategies for detection of biomarkers. The biomarker PSA (prostate specific antigen), which is prostate cancer specific, has been the main focus of the thesis. LÄS MER

3. 2. Biomarker detection via lab-on-a-chip integrated immunoaffinity approach for fluorescence and mass spectrometry readout

   Författare :Asilah Ahmad Tajudin; Avdelningen för Biomedicinsk teknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; immunoaffinity; affinity prefractionation; immunoMALDI; solid-phase extraction; protein digestion; ISET; antibody microarray; signal amplification; fluorescence; microfluidics; lab on a chip; separation; acoustophoresis; ultrasound; acoustic radiation force; plasma; proteomics; MALDI mass spectrometry; immunoassay; porous silicon.;

   Sammanfattning : The blood/plasma protein biomarker profile represents the pathological and physiological changes relating to human diseases. However, the plasma proteome reflects a high degree of complexity that results in a challenge for most analytical methods in clinical diagnostics. LÄS MER

4. 3. High-throughput protein analysis using mass spectrometry-based methods

   Författare :Tove Boström; Sophia Hober; Thomas Laurell; Ole N. Jensen; KTH; []
   Nyckelord :Proteomics; mass spectrometry; affinity proteomics; immunoenrichment; immunoprecipitation; IMAC; screening; protein production; protein purification; ISET; quantification; SILAC; stable isotope standard; antibody validation; Bioteknologi; Biotechnology;

   Sammanfattning : In the field of proteomics, proteins are analyzed and quantified in high numbers. Protein analysis is of great importance and can for example generate information regarding protein function and involvement in disease. Different strategies for protein analysis and quan- tification have emerged, suitable for different applications. LÄS MER

5. 4. High-throughput screening of solid-phase extraction materials using mass spectrometry

   Författare :Kishore Jagadeesan; Avdelningen för Biomedicinsk teknik; []
   Nyckelord :mass spectromery; protein separation; sample preparation; Molecularly imprinted polymer; Data analysis; Data visualization; Miniaturisation; Proteomics;

   Sammanfattning : In biomarker analysis, sample preparation plays a crucial step in order to extract, isolate and concentrate the analytes of interest. Among the various sample preparation techniques, solid phase extraction (SPE) is one of the most common and popular, due to the high enrichment factor, good recovery, low consumption of organic solvents and the possibility to automate (off- or on-line) the whole process. LÄS MER