Sökning: "ferrochelatase"

Visar resultat 1 - 5 av 12 avhandlingar innehållade ordet ferrochelatase.

  1. 1. Crystallographic studies on porphyrin metallation by ferrochelatase

    Författare :David Lecerof; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; thermal and mechanical properties; Condensed matter:stucture; fasjämvikt; kristallografi; egenskaper termiska och mekaniska ; Kondenserade materiens egenskaper:struktur; crystallography; phase equilibria; Magnesium; pi-helix; Zinc; ferrochelatase; Metal binding; porphyrin metallation;

    Sammanfattning : Ferrochelatase catalyses the terminal step in heme biosynthesis by inserting a ferrous ion into protoporphyrin IX. The reaction mechanism has in this thesis been studied by mainly crystallographic methods. LÄS MER

  3. 2. Ferrochelatase and Magnesiumchelatase: Metal chelation studied with mutants

    Författare :Ulf Olsson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; chlorophyll; heme; barley; mutants; Magnesium chelatase; ferrochelatase; Metabolism; Biokemi; metabolism;

    Sammanfattning : Magnesium chelatase and ferrochelatase are two very important enzymes, which are involved in chlorophyll and heme biosynthesis, respectively. They both use protoporphyrin IX as substrate but magnesium chelatase inserts a magnesium ion whereas ferrochelatase inserts a ferrous ion. These two enzymes have been studied in this thesis. LÄS MER

  4. 3. Iron in Haem Biosynthesis. Structural Studies of Ferrochelatase and Frataxin

    Författare :Tobias Karlberg; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Naturvetenskap; Natural science; Molekylär biofysik; Molecular biophysics; Biomedicinska vetenskaper; Biomedical sciences; protein crystallography; ferrochelatase; haem biosynthesis; iron; frataxin;

    Sammanfattning : Ferrochelatase catalyses the synthesis of heme by inserting Fe(II) into protoporphyrin IX. The structure of ferrochelatase from yeast has been determined (2.4 Å). The structure is homodimeric with each of the monomer made up of two Rossmann type domains with an active-site cleft between them. LÄS MER

  5. 4. Structural and physicochemical studies on metal and porphyrin binding by the Bacillus subtilis ferrochelatase

    Författare :Mattias Hansson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; metal specificity; metal binding; porphyrin metallation; time resolved crystallography; fluorescence quenching; X-ray structure; inclusion bodies; Bacillus subtilis; heme biosynthesis; ferrochelatase; hemH;

    Sammanfattning : The enzyme ferrochelatase (EC 4.99.1.1) is the terminal enzyme in the biosynthetic pathway of heme b and catalyzes the insertion of Fe(II) into protoporphyrin IX. LÄS MER

  7. 5. Theoretical studies of porphyrin proteins

    Författare :Emma Sigfridsson; Beräkningskemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; molecular mechanics; quantum chemical calculations; electrostatic potential charges; atomic charges; ferrochelatase; cytochrome; myoglobin; Porphyrin; haem; QM MM; reorganisation energy; Theoretical chemistry; quantum chemistry; Teoretisk kemi; kvantkemi;

    Sammanfattning : Different aspects of porphyrin proteins have been studied with theoretical methods. For example, we have studied: - The importance of hydrogen bonds for the discrimination between carbon monoxide and molecular oxygen by myoglobin, the oxygen carrier in muscles, modelling the active site both in vacuum and in the protein matrix - The inner-sphere reorganisation energy during electron transfer for cytochromes compared to other electron-transfer proteins, such as blue copper proteins and Fe-S clusters - The role of porphyrin distortions in the reaction mechanism of ferrochelatase, the enzyme that inserts an iron ion into protoporphyrin IX to make haem - The interaction between different metal sites in ferrochelatase In these studies we have used density functional theory, classical force field methods, and a combination of quantum chemistry and molecular mechanics. LÄS MER